Table 4. Experimental Setup of Assay Kit Components Note: (1)“—” No requirement ; Ct>37 or No Ct, Not detected.diagnosis 1. Limitation of Detection (LOD): 100 copies/mL. 2. Internal precision: repeatability: CV< 10%, between-run precision: CV < 10%, between-day precision: CV< 10%, total precision: CV< 10%. 3. External precision: repeatability: CV< 10%, between-run precision: CV < 10%, total precision: CV < 10%. 4. Interference reaction: Five potential reference (Dexamethasone, Azithromycin, Tobramycln, Levofloxacin, Ceftriaxone) will not interfere with the detection results of the kit. 1. Negative results cannot completely rule out the existence of infection of Monkeypox viruses. Improper sample collection, improper transportation, improper processing and insufficient initiation VL (viral load) may influences the experimental results. 2. Other unverified interferences or PCR inhibitors may cause false negative [Warnings and Precautions] 1. For in vitro diagnostic use only. 2. Carefully read this instruction before use. Components from different batch number cannot be used interchangeably.

3. After being reconstituted, the lyophilized components can be either used up or stored at -20±5°C and can be kept for one month. Avoid repeated freeze- thaw for more than five times. 4. RT-PCR Master Mix are sensitive to temperature and should always put on ice during experiment. 5. Always wearing gloves and mask during experiment to avoid microbial and nuclease (DNase/RNase) contamination of the specimen and the reagents of the kit. 6. Always use DNase/RNase-free disposable aerosol-blocking pipette tips. 7. Additional controls may be tested according to guidelines or requirements of local,...