Access Array
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FLUIDIGM SPECIFICATION SHEET PN 100-4161 D1 Access Array • Accuracy: >90% mapping to target, >85% mapping to genome • Yield: 480 targets across 48 samples with up to 384 unique sample indexes • Simplicity: 12 pipette steps, 20 minutes hands-on time, 5hrs to a sequence ready library The Access Array™ system is a unique target enrichment platform designed for sequencing selected regions from large numbers of samples. The system combines the cost and throughput benefits of Fluidigm® integrated fluidic circuits (IFCs) with the proven performance and flexibility of PCR. Obtain quality results while minimizing the time, cost and labor required for targeted sequencing projects. The Access Array system is an open platform allowing for a diverse primer design strategy through D3 Assay Design or our Assay Design Group. TARGET ENRICHMENT Target enrichment using the Access Array System combines thermal mixing with an optimized PCR protocol for unparalleled uniformity across the target regions. SAMPLE BARCODING FOR MULTIPLEXED SEQUENCING One of the greatest challenges facing next-generation sequencing today is how to use the massive amounts of throughput enabled by the new generation of sequencers. Fluidigm Access Array barcodes provide a simple and reliable method for running multiple samples per sequencing run. Barcoding samples during the target enrichment process enables the users to pool multiple samples per sequencing run, and simplifies the sample de-multiplexing during the data analysis step based on the barcode. LIBRARY PREPARATION USING AMPLICON TAGGING Library preparation for next-generation sequencing is the foundation for a successful targeted sequencing run. However, it can also be the biggest source of error, or contamination in the workflow. While necessary, the process can be streamlined by encapsulating a PCR amplicon tagging strategy that incorporates the Fluidigm universal CS tags with the target specific primers into the 48.48 Access Array IFC.

ACCESS ARRAY TARGET-SPECIFIC PRIMERS Fluidigm Access Array target-specific primers and the Access Array system allows researchers to rapidly and cost-effectively pursue highsample throughput amplicon sequencing studies to better understand genetic or genomic variation. Access Array target-specific primers are customdesigned to optimize coverage and performance. When used with the Access Array system, these primers allow for fast, simple and inexpensive preparation of up to 480 amplicons across 48 samples in a single Access Array IFC run. Our sample barcode sequences allow up to 384 unique...

The Access Array system is compatible with multiple system components to meet a variety of application and sample throughput needs. IFC CONTROLLER COMPATIBILITY Parameter Targeted Resequencing Experiment tracking Gas pressure Interface IFC Controller AX software Dimensions (approx.) IFC Controller AX 48.48 Access Array IFC Barcode Internal compressor USB and ethernet Touchscreen interface for operating and tracking 19 x 9.5 x 13 inches; 48.5 x 24 x 33 cm THERMAL CYCLER COMPATABILITY Parameter Targeted Sequencing Connectivity Interface Dimensions (approx.) FC1 Cycler 48.48 Access Array IFC...

• System Components Each system has two IFC controller AX’s and a single FC1 cycler. The complete system allows you to load the 48.48 Access Array IFC, amplify and tag the regions of interest and recover the product. • 48.48 Access Array IFC Unique IFC designed to multiplex 48 samples against 48 assay reactions in 35 nL reaction volumes. The 48.48 Access Array IFC prepares 2,304 individual reactions at a time with less than 50 ng of template DNA. • Access Array Barcode Library Use the 48.48 Access Array IFC with a set of 48 barcodes integrated with sequencing adaptors to pool the PCR...