FLUIDIGM PN 101-0723 A1 SPECIFICATION SHEET Helios A CyTOF® system Helios™ brings high-parameter capabilities to protein research, expanding the breadth and depth of phenotypic and functional profiling in single cells. Helios technology addresses the challenges of high-parameter, single-cell analysis, providing researchers with tools to dissect the complex proteomes of cell systems in normal and diseased states. Helios analyzes individual cells labeled with stable metal isotopes using state-of-the-art Helios Specifications* Description Specification Instrument response 600,000 counts/pg Tb Detection limit 350 antibodies/cell Dynamic range 4.5 orders of magnitude Calibration Automated Operating system Windows® 7 Pro 64-bit Data storage 7.2 TB RAID (mirrored) Sample introduction Pneumatic single tube loader with agitation, up to 5 mL volume Peak throughput 2,000 events/sec Flow rate 30 ^L/min inductively coupled plasma time-of-flight (TOF) technology. With 135 detection channels, Helios can simultaneously resolve multiple elemental probes at high acquisition rates, thereby maximizing the per-cell information obtained from a single sample. The system’s newly designed pneumatic sample introduction system and its new software interface make mass cytometry more accessible than ever. Description Specification Dimensions Width 41 in (103 cm) Height 52 in (132 cm)

Workstation Specifications* Operating system Specification Width Hard drive Data storage Autosampler (optional)* Description Sample volume Sample resuspension Wash solution Probe pipet motion Reagent reservoirs Carrier (DIW)  x -well plate, up to  mL deep well File Type Sample format Data File Size * Specifications for Helios, components and accompanying computer system are subject to change without notice. CORPORATE HEADQUARTERS 7000 Shoreline Court, Suite 100 South San Francisco, CA 94080 USA Toll-free: 866 359 4354 | Fax: 650 871 7152 fluidigm.com SALES North America | +1 650 266 6170...