Hyperion Imaging System
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Hyperion Imaging System Visualize a new path forward

Changing the course of how disease is treated and ultimately cured requires a comprehensive understanding of complex cellular phenotypes and their interrelationships in the spatial context of the tissue microenvironment. To meet this great challenge, we developed the Hyperion™ Imaging System. The Hyperion Imaging System enables you to uncover new biomarkers and cellular interactions by using Imaging Mass Cytometry™. With the capability to simultaneously interrogate 4 to 37 protein markers at subcellular resolution without spectral overlap or background autofluorescence, you can gain...

DAVID RIMM, MD, PHD Professor of Pathology Yale University School of Medicine

“RESEARCH IN MY LAB IS RELATED TO BIOMARKERS that either predict outcome or response to therapy or to a drug. I see the Hyperion Imaging System as a valuable discovery tool that can provide information about which molecules are most important to predict response to therapy.”

Translational and clinical researchers are now applying this groundbreaking technology to make new advances across a breadth of disease research areas. The system design enables highly multiplexed immunohistochemistry (IHC) to provide a comprehensive view of the tissue microenvironment, making it possible to perform in-depth characterization of protein biomarkers, cellular composition and signaling pathways. With cell segmentation and analytical tools, researchers can illuminate changes in unique cellular phenotypes and their functional states—changes that may correlate with disease...

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A SIMPLE AND COMPLETE SOLUTION FOR MULTIPLEXED IMAGING WORKFLOW The four-step Imaging Mass Cytometry workflow (Figure 1) enables deep profiling of standard formalin-fixed, paraffin-embedded (FFPE) or frozen tissue sections and fixed cells using the Hyperion Imaging System. With steps familiar to researchers currently using IHC or other fluorescence imaging approaches, this simple workflow can run unattended to image tissue samples.

IMAGING MASS CYTOMETRY WORKFLOW 2. STAIN Stain tissues (FFPE or frozen) or fixed cells using familiar IHC protocols. 1. DESIGN Design panels using pathologist-verified Maxpar® antibodies conjugated to metal tags. 3. IMAGE Image protein markers at subcellular resolution using the Hyperion Imaging System. 4. ANALYZE Analyze images in minutes using MCD Viewer and easily export for secondary analysis. FIGURE 1 The imaging Mass Cytometry workflow is a simple four-step workflow to deliver highly multiplexed imaging.

KLAUS KAESTNER, PHD, MS Thomas and Evelyn Suor Butterworth Professor in Genetics University of Pennsylvania Perelman School of Medicine

“THROUGH THE ABILITY OF LOOKING AT 35 different antigens in a tissue section and their relationship to each other, we can really get new insights about cell heterogeneity-cell interactions—that we weren’t able to even approach before.”

Expanding beyond the inherent technical limitations of fluorescence-based IHC, the Hyperion Imaging System uses proven CyTOF technology to simultaneously detect 4 to 37 protein markers from cells and tissues using highly pure metal isotopes conjugated to antibodies. Traditional fluorescence is restricted to detection of only a few markers at a time because of spectral overlap of fluorescent antibody tags, variable staining intensities and noise from autofluorescence or other background fluorescence (Figure 2). The Hyperion Imaging System uses metal-tagged antibodies instead of fluorophores....

ALL AT ONCE Visualize 4-37 individual protein markers from a single scan to uncover important phenotypes and relationships.

Relative Intensity (%) Relative Intensity (%) A NEW STANDARD FOR HIGH-MULTIPLEX PROTEIN DETECTION A. Fluorescence signal overlap Hoechst FITC ■ Cy3® ■ Texas Red™ «Cy5® B. CyTOF technology results in separate and distinct peaks. FIGURE 2 CyTOF technology offers a new standard for high-multiplex protein detection. (A) Fluorescence spectra provide information about the excitation and emission of fluorophores. Shown are the emission spectra for a selection of commonly used fluorophores, with relative intensity of each fluorophore set at 100%. Each fluorescent dye has a characteristic emission...

BERND BODENMILLER, PHD Assistant Professor Institute of Molecular Life Sciences, University of Zurich

“NOW THAT WE CAN MEASURE SO MANY MARKERS simultaneously, for the first time we have the ability to comprehensively study cell types and their signaling and functional state in normal tissue and especially in tumors, and also how they are spatially arranged.”

DEEPLY PROFILE CELLS AND TISSUES IN SPATIAL CONTEXT-ALL AT SUBCELLULAR RESOLUTION Each pulse samples the tissue and associated protein markers from a 1 urn2 tissue spot for comprehensive analysis of the selected region of interest (Figure 3). A plume formed from each spot, which is a cloud of particles that contains tissue and metal-tagged antibodies bound to the protein epitopes, is ionized and detected using CyTOF technology. Following detection, the tissue is moved by 1 um and the next pixel is sampled. For each spot, identified with pre-determined coordinates, multiple metal tags are...

HOW IMAGING MASS CYTOMETRY WORKS Load sample into the Hyperion Imaging System. Precise laser imaging of the region of interest Load the slide stained with a panel of Maxpar antibodies into the Hyperion Imaging System. A laser beam focused at 1 μm2 spots samples proteins stained with metal-tagged antibodies Select the region of interest to be imaged. and directs these tags to analysis by inductively coupled time-of-flight (TOF) technology, the basis for CyTOF mass cytometry. This occurs in a single scan as the laser samples each 1 μm2 pixel in the selected region.

These samples often require analysis of a multitude of markers, yet tissue input is often limited. This is especially true when using small biopsy samples such as fine needle aspirates or samples that need to be preserved for additional analysis. Ideal for deep profiling of your precious FFPE and fresh frozen tissue samples, the Hyperion Imaging System eliminates the variability that comes from using serial sections or sequential staining and reduces the inherent variability that comes from changing tissue architecture across serial sections. With this system you can get superior...