FLUXION BIOSCIENCES DATA SHEET IsoFlux Cytation Imager Fully integrated imager for automated scanning and detection of CTCs from the IsoFlux System The IsoFlux Cytation™ Imager is designed specifically for CTC (circulating tumor cell) enumeration and protein detection by fluorescent imaging. Based on the industry-leading Cytation imagers from BioTek Instruments, Inc., the IsoFlux Cytation Imager comes pre-configured with the filters, objectives, and software configurations necessary for automated scanning of IsoFlux™-generated CTC samples. The IsoFlux Cytation Imager integrates easily with the IsoFlux CTC enrichment and enumeration workflows, allowing automated CTC enumeration without the complexity of traditional inverted microscopes. Software comes pre-configured for CTC image acquisition using the IsoFlux CTC Enumeration Kit Performs scanning and identification of putative CTCs in less than 10 minutes per sample Streamlined user review of potential CTCs reduces the overall time per sample by 3X Includes 4X and 10X objectives for scanning and high resolution image capture Automated XY stage, auto focus, auto exposure, auto image capture Includes fluorescent filter cubes for RFP, GFP, and DAPI for CTC detection and enumeration Available fourth fluorescent channel for additional protein marker detection (e.g., AR-V7, etc.)

DATA SHEET FLUXION BIOSCIENCES Specifications General Part number Imaging modes Labware type 930-0375 Fluorescence and bright field Protocol optimized for CTC enumeration using Greiner 24-well plates Gen5™ Data Analysis Software. Preconfigured image acquisition using the IsoFlux CTC Enumeration Kit; Preconfigured CTC identification protocols Software Analysis Imaging Camera Imaging filter capacity Included imaging filter cubes: Optional 4th channel filters for additional protein detection Light source Objective capacity Included objectives Optional objectives Autofocus CTC Identification 16-bit...