IsoFluxTM System
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IsoFluxTM System - 1

The next generation of CTC Analysis is here

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IsoFluxTM System - 2

PRODUCT OVERVIEW IsoFlux System The next generation of circulating tumor cell analysis is here The IsoFlux System enriches intact rare cells from biological samples and prepares them for further analysis. It gives you immediate access to highquality, viable cells in a high-density format, ready for use in the application of your choice.

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IsoFluxTM System - 3

-\ Benefits Access a Wide Range of Samples - Process multiple starting sample types including whole blood, fractionated blood, dissociated tissue, and cell cultures. Use Your Own Capture Criteria - Capture cells using single or multiple capture antibodies. Use pre-validated or user-defined kits. Capture Intact, Pure Cells - Recover target cells with high yield and purity using minimal processing. Minimize Sample Dilution - Gain immediate access to highly concentrated, low-volume samples ready for molecular and cell-based analysis. Save Time - Increase your lab’s productivity with the...

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IsoFluxTM System - 4

The CellSpot™ Advantage The IsoFlux System uses proprietary CellSpot Technology to deliver enriched cells in a format ideal for next generation molecular applications. CellSpot increases the purity, integrity, and yield of isolated cells, while also making them easy to access for use in downstream applications. How It Works 1. Sample Loading A biological sample is prepared with magnetic particles. The sample is loaded into the inlet of the IsoFlux microfluidic cartridge. Up to 4 cartridges can be loaded into the instrument at one time. The CellSpot Cap is loaded into the cartridge that has...

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IsoFluxTM System - 5

Go from sample to analysis faster than you ever thought possible. Sample Collection • 7-10mL blood draw • EDTA • Overnight shipment Sample Preperation • 30 min. hands on time • 60 min bead coupling CTC Isolation • 45 min. inside instrument • Fully automated Sample Ready • Sample ready fo

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IsoFluxTM System - 6

SYSTEM VALIDATION Performance where you need it. The IsoFlux System delivers high quality CTC enrichment with consistent, reliable performance. Validation testing has demonstrated superior CTC recovery across a wide range of pathologies. Graph Number #1 Superior transferability to downstream analysis High recovery of CTCs is critical to ensuring enough cellular material to perform downstream analysis from a high enough percentage of patients. In this experiment, a prostate cancer cell line (PC3) was spiked into samples of 7mL healthy donor blood (N=36) at varying concentrations, ranging...

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IsoFluxTM System - 7

DOWNSTREAM APPLICATIONS Mutation Analysis Mutations in genes such as KRAS, EGFR, and BRAF act as molecular switches that can interfere with normal cell regulation. Identification of these mutations can offer insight for how a patient will respond to particular therapies. CTCs recovered from IsoFlux samples can be lysed for DNA recovery and tested for mutations using qPCR, dPCR, or NGS. In this example, a KRAS mutation is detected in a colorectal cancer patient using qPCR. Gene Expression Expression profiling is often performed using RT-qPCR when there are many genes of interest in the...

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IsoFluxTM System - 8

IsoFlux System Specifications Dimensions: 16in wide x 22in tall x 25in deep (40cm x 56cm x 64 cm) Weight: 120lbs (60kg) Throughput: 16 samples/day (4 in parallel) Temperature control: 4°-8° C Power supply: 110-220V (autoranging) Touch screen operation 1 year warranty Catalog No: 950-0100 For In Vitro Diagnostic Use IsoFlux Circulating Tumor Cell Enrichment Kit Number of samples processed: 8 Cell enrichment method: immunomagnetic beads Cell enrichment reagent: anti-EpCAM (targeted towards circulating cells of epithelial origin) Kit contents: 8 microfluidic cartridges, immunomagnetic...

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