ÄKTA pilot 600
5Pages

Catalog excerpts

Flow accuracy and gradient performance of ÄKTA pilot 600 Intellectual Property Notice: The Biopharma business of GE Healthcare was acquired by Danaher on 31 March 2020 and now operates under the Cytiva™ brand. Certain collateral materials (such as application notes, scientific posters, and white papers) were created prior to the Danaher acquisition and contain various GE owned trademarks and font designs. In order to maintain the familiarity of those materials for long-serving customers and to preserve the integrity of those scientific documents, those GE owned trademarks and font designs remain in place, it being specifically acknowledged by Danaher and the Cytiva business that GE owns such GE trademarks and font designs. cytiva.com GE and the GE Monogram are trademarks of General Electric Company. Other trademarks listed as being owned by General Electric Company contained in materials that pre-date the Danaher acquisition and relate to products within Cytiva’s portfolio are now trademarks of Global Life Sciences Solutions USA LLC or an affiliate doing business as Cytiva. Cytiva and the Drop logo are trademarks of Global Life Sciences IP Holdco LLC or an affiliate. All other third-party trademarks are the property of their respective owners. © 2020 Cytiva All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most current information. For local office contact information, visit cytiva.com/contact

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Flow accuracy and gradient performance of ÄKTA™ pilot 600 Accurate flow is important for your chromatography step to provide reproducible results at different scales. For ÄKTA pilot 600 chromatography system, flow and gradient formation is controlled by the UNICORN™ system control software. In this work, flow accuracy was investigated at a combination of different backpressures, flow rates, temperatures, and liquid viscosities. Gradient accuracy and range were calculated from the flow accuracy. Gradient accuracy was also evaluated by running step and linear gradients at different flow...

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If a system instead has a flow accuracy specified as % RD, the error will always be the same percentage of the read value. In such a case, with a system accuracy of 2% RD, the deviation would be ± 20 mL/min at a flow rate of 1000 mL/min, but only ± 2 mL/min at 100 mL/min. Designation of gradient accuracy Gradient accuracy is often presented as a gradient acceptance range, with a defined accuracy within the acceptance range or as a chromatogram over a programmed and a performed gradient (Fig 2). The gradient acceptance range is given as % of buffer B (%B) for the flow rate range of the...

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Conclusion This work demonstrates the flow and gradient accuracy of ÄKTA pilot 600. The flow accuracy of ÄKTA pilot 600 was found to be ± 2% RD. Gradient deviation was found to be less than 1%B within the gradient range from 1%B to 99%B at a system flow rate of 5–600 mL/min. The high accuracy over a multitude of parameters such as flow rate, viscosity, and pressure indicates that the system has a highly stable performance, omitting the need of a separate flowmeter to feedback control the pumps. The high accuracy, together with small hold-up volumes, strong sanitary performance (1), and a...

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GE Healthcare Bio-Sciences AB Björkgatan 30 751 84 Uppsala Sweden GE, the GE Monogram, ÄKTA, and UNICORN are trademarks of General Electric Company. Any use of UNICORN software is subject to GE Healthcare Standard Software End-User License Agreement for Life Sciences Software Products. A copy of this Standard Software End-User License Agreement is available on request. © 2018 General Electric Company All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on...

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ÄKTA pilot 600 5Pages

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ÄKTA pilot 600
5Pages