ÄKTApurifier
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GE Healthcare Data file 18-1119-48 AF Chromatography systems ÄKTApurifier core systems (Fig 1) are versatile, modular liquid chromatography systems for fast and reliable separations of proteins, peptides, and nucleic acids by TM FPLC . The four available core systems can be combined TM with ÄKTAdesign automation kits and hardware to achieve tailor-made solutions for your protein purification needs. Using ÄKTAdesign automation kits to expand ÄKTApurifier core systems also eliminates a number of common problems in FPLC purification, such as long sample loading time, frequent column cleaning, and air corrupting the system. • Four core system configurations for greater versatility • High-performance purification by FPLC • System configurations designed to meet individual research needs • ÄKTAdesign automation kits can be added to provide greater flexibility • Greater flexibility keeps costs down – you only buy what you need Wide range of purification scales ÄKTApurifier 10 and UPC 10 configurations are designed for purification of microgram to milligram quantities of protein, operating at flow rates of 0.001 to 10 ml/min at pressures of 0 to 25 MPa. ÄKTApurifier 100 and ÄKTApurifier UPC 100 systems can purify gram quantities of protein, running at flow rates of 0.01 to 100 ml/min and pressures of 0 to 10 MPa. UNICORN control ÄKTApurifier systems are part of ÄKTAdesign, a range of liquid chromatography systems for academic and industrial research laboratories. ÄKTAdesign systems are controlled by TM UNICORN , a control, evaluation, and reporting platform. UNICORN ensures quick, simple communication between systems and users and meets the stringent control and data handling procedures of modern laboratories. Optimized methods are transferred easily to production scale. Fig 1. ÄKTApurifier UPC (above) and ÄKTApurifier (below)

Easy access in the lab ÄKTApurifier systems require minimal bench space, with each system mounted on a swivel platform for easy access to every side. Columns are snapped in place on the outside of the system, either individually or as groups held in column holders. Both pumps enable accurate, reproducible flow and gradient formation over a wide flow rate range (Fig 3). The pumps can be used for loading samples larger than 50 ml, but for easy, routine handling of large sample volumes, Sample Pump P-960 is recommended. 25 System configurations provide flexibility • Buffer selection kit •...

ÄKTApurifier UPC 10 and 100 core systems include Monitor UPC-900, which combines monitoring of UV absorption, pH, and conductivity. The monitor features fixed wavelength of 254, 280 nm (Hg lamp) or 214 nm (Zn lamp). Other wavelengths can be measured by changing the filter. Two alternative flow cells with path lengths of 2 mm and 5 mm are available. Improved system functionality with ÄKTAdesign automation kits All four ÄKTApurifier core systems can be expanded using an extensive range of ÄKTAdesign automation kits and hardware, which enable customization of systems to suit different...

BufferPrep can be used for pH scouting in rapid method optimization. The UNICORN method Wizard makes programming of automatic eluent pH scouting for ion exchange chromatography easy and reliable. Air sensor Kit The Air Sensor Kit enables safe exclusion of air from the ÄKTApurifier system during the purification. The components of Air sensor Kit are controlled by UNICORN software, which detects air in the system, and pauses the purification run. The kit therefore prevents column damage and ensures that the sample is completely injected. In addition, UNICORN is programmed to ensure that a...

Reliable system control with UNICORN Autosamplers give you more time to work on other projects during the day, and increase throughput by enabling overnight purification runs. Depending on your needs for sample injection, Autosampler A-900 and A-905 for ÄKTAdesign systems offer a variety of alternatives. These autosamplers offer different balances between reproducibility and accuracy and allow flexible use of vials and microplates. Almost every type of vial with an o.d. of 12 mm or 7 mm can be used on Autosampler A-900. Autosampler A-905 handles standard and deep-well 96-well microplates,...

Start Prdtocd | Fiac-WO fv Dfahp totffa icu fJwdid mailing -ictwftt c<h Minimal procedure times User selected and defined UNICORN watch functions (Fig 10) are entered into a method to instruct specific events to occur when preset conditions are reached. For example, the watch function will wait for a return to normal baseline level before instructing the next run to begin, minimizing the time spent on column re-equilibration between runs. Fig 8. Automatic scouting during purification development and optimization. The example shows scouting for flow rate, sample volume, Accurate result...

UNICORN networks UNICORN control system has full networking capability, with high security and maximum compliance with regulatory needs. Network support allows control of any ÄKTAdesign system from any connected UNICORN workstation. System control and data access via a PC network is extremely easy to use and gives a complete overview of operations. 250 μl of clarified estradiol (E2)-treated vitellogenin (Vtg) fish 250 µl clarified estradiol (E2)-treated plasma sample vitellogenin (Vtg) fish plasma sample Start buffer: 100 mM Tris-HCl, 1 mM PMSF, pH 7.0–8.5 Start buffer: 0.1 M Tris-HCl, 1mM...

System specifications ÄKTApurifier Flow rate range setting, ÄKTApurifier 10 Pressure range, ÄKTApurifier 10 Flow rate range setting, ÄKTApurifier 100 Pressure range, ÄKTApurifier 100 Conductivity range (RPC – IEX – HIC gradients) pH range Temperature range Solvent compatibility Size (W × H × D) Weight 0 to 25 MPa 0.01 to 100 ml/min 0 to 10 MPa 1 µS/cm to 999.9 mS/cm 2 to 12 4°C to 40°C all commonly used chromato­graphic solvents 500 × 620 × 460 mm 75 kg Operating data Pump P-903 Flow rate range Increment Pressure range Pressure limits Internal volume Viscosity 0.001 to 10 ml/min 0.001...