Amersham Imager 600
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Imaging systems, software, and accessories Amersham™ Imager 600 Amersham Imager 600 series is a new range of sensitive and robust imagers for the capture and analysis of high resolution digital images of protein and DNA samples in gels and membranes. These multipurpose imagers bring high performance imaging to chemiluminescence, fluorescence, and colorimetric applications. The design of Amersham Imager 600 combines our Western Blotting application expertise with optimized CCD technology and exceptional optics from Fujifilm™. The system has an integrated analysis software and intuitive workflow, which you can operate from an iPad™ or alternative touch screen device, to generate and analyze data quickly and easily. Amersham Imager 600 delivers: • Intuitive operation: You can operate the instrument from a tablet computer with an intuitive design and easy-to-use image analysis software. You do not need prior imager experience or training to obtain high-quality results. Use the automatic capture mode for convenient exposure • Excellent performance: The system uses a superhoneycomb CCD and a large aperture f/0.85 FUJINON™ lens, which consistently delivers high-resolution images, high sensitivity, broad dynamic range (DR), and minimal cross-talk • Robustness: Combining minimal maintenance with our proven expertise in Western blotting and electrophoresis makes the imager well suited for multiuser laboratories. Amersham Imager 600 is an upgradable series of imagers that can grow with your imaging needs Description Amersham Imager 600 series is equipped with a dark sample cabinet, a camera system, filter wheel, light sources, and a built-in computer with control and analysis software. Network connection and USB ports are standard (Fig 2). Fig 1. Amersham Imager 600 series is a range of robust and easy-to-use systems for chemiluminescent, colorimetric, and fluorescent image capture. Settings such as focus, filter, illuminator, and exposure type are automatically controlled by the integrated software. You would obtain high resolution images and precise quantitation of low signals with the multipurpose 16-bit 3.2 megapixel camera fitted with a large aperture lens. The detector is cooled to reduce noise levels for high sensitivity and wide dynamic range. Rapid cooling leads to a short startup time, which makes the instrument ready to use in less than 5 min. You can place the sample tray at one of two different heights in the sample compartment to produce image-acquisition areas of 220 × 160 mm and 110 × 80 mm, respectively.

The system can be used for a wide range of applications and it is fully upgradable between four different configurations (Table 1). Each configuration can be used for chemiluminescent detection and nonquantitative gel documentation. The different configurations are equipped with light sources and filters for UV and white light trans-illumination, and red, green and blue epi-illumination for multiple fluorescence detection. Optica density (OD) measurements are calibrated for quantitation of colorimetric staining applications. You can operate the system via an integrated computer, controlled...

Imaging performance Intuitive operation and analysis A bright, wide aperture FUJINON f/0.85 lens developed for chemiluminescent imaging projects sharp images onto a specially patterned CCD (Fig 3). Amersham Imager 600 can be controlled from either an iPad or an alternative touch screen device. The user interface is intuitive and the workflow is easy to follow. The system is fully automated, which means that after startup, you do not need to perform focusing, insertion of light sources, changing of filters, calibrations, or other adjustments. When the system is in automatic image capture...

Sample: NIH/3T3 cell lysate two-fold dilution series starting at 5 µg Membrane: Amersham Hybond™ P Blocking: Amersham ECL Prime blocking agent 2% in PBS-T Primary antibody: Rabbit anti-ERK1/2 1:10 000 Secondary antibody: ECL Anti-rabbit IgG horseradish peroxidase 1:100 000 Detection: Amersham ECL Select Imaging: Amersham Imager 600 Imaging method: Chemiluminescence Dynamic range: 2.7 orders of magnitude 1.5 2.5 3.5 Log cell lysate amount (ng) Fig 4. A two-fold dilution series of NIH/3T3 cell lysate starting at 5 µg total protein was subjected to chemiluminescent Western blotting and ERK was...

Sample: Blocking: Primary antibody: Secondary antibody: Imaging: Imaging method: E. coli lysates 3% BSA in PBS-T Rabbit anti DHFR C-terminal 1:1000 ECL Plex Goat anti rabbit-Cy3 IgG 1:2500 Amersham Imager 600 Fluorescence Cy3, Cy5 Prelabeling Cy5 Total protein Sensitive imaging of total protein stains Proteins may be visualized by treating a gel with a total protein stain after performing 1D or 2D electrophoresis. The most commonly used stains are Coomassie Blue or silver staining. Fluorescent staining methods such as SYPRO™ Ruby protein gel stain have the advantage of being more sensitive....

Electrophoretic separation of DNA is a common technique that is typically used for the analysis of vector cleavages, DNA purification, and verification of successful PCR. Traditionally, ethidium bromide (EtBr) has been used for visualizing DNA, but today there are many alternative DNA stains available, such as SYBR™ Green. Amersham Imager 600 QC is a dedicated configuration for densitometry applications in a QC environment. The system contributes to a reliable control of products because it is equipped with highly sensitive optics that can detect trace amounts of impurities accurately....

Technical features Ordering information Table 2. Amersham Imager 600 RGB specifications Code number Peltier cooled Fujifilm Super CCD Pixel area Lens model: Two-stage thermoelectric module with air circulation Accessories included (depending on configuration) Cooling down time: Dynamic range: White trans Tray AI600 Image resolution: Fully automated (auto exposure, no focus or other adjustment or calibration needed) Additional Accessories Gel sheets (for UV trans tray) iPad 4 with AC Adapter Kit Touch Screen Monitor with Stand Pixel correction: Dark frame correction, flat frame correction,...