Imaging systems, software, and accessories Amersham™ Typhoon™ Biomolecular Imager Amersham Typhoon Biomolecular Imager (Fig 1) is a new generation of laser scanners that provide you with exceptional data quality through extremely sensitive detection, high image resolution, and a very broad linear dynamic range. These versatile imaging systems support multiple imaging modes, including phosphor imaging, red/green/blue (RGB) and long and short wavelengths of near infrared fluorescence (NIR), as well as optical densitometry (OD) of proteins in stained gels. The Amersham Typhoon 5 model offers a five-laser configuration option with advanced photomultiplier tubes to cover all of these imaging modes. Four other Amersham Typhoon models are available—one for RGB fluorescence/OD measurement/phosphor imaging, one for NIR short/NIR long/Green fluorescence, one for NIR short/NIR long fluorescence, and one for phosphor imaging— so you can choose the best option based on the needs of the system users. Moreover, upgrade paths among different models are available at any time after the installation. Amersham Typhoon scanners deliver: • Versatility: use one system to image multifluorescent-, radioisotope-labeled, and colorimetric samples on gels, membranes, multiwell plates, culture dishes, glass slides, and tissue sections. The IP model is for phosphor imaging only but can be upgraded • Accurate quantitation: detect signals from as low as 3 pg of protein and differences across a dynamic range with greater than 5 orders of magnitude Fig 1. Amersham Typhoon Biomolecular Imagers are versatile, highperformance laser scanners for sensitive and quantitative measurements in a multiuser environment. The image shows the main instrument (right), the Amersham Eraser (top left), and the accessory cabinet (bottom left). • Flexibility: modular design allows you to customize the imager for your users’ needs. Systems can be adapted with stages, detectors, filters, and lasers. Several upgrade kits are available • Ease of use: Amersham Typhoon 5, RGB, NIR Plus, and NIR models have auto- and semi auto-scan functions, as well as automatic filter recognition The Amersham Typhoon series of scanners provides you with versatile and flexible imaging to precisely quantitate proteins, nucleic acids, and other biomolecules. Amersham Typhoon 55, Amersham Typhoon RGB, Amersham Typhoon NIR Plus, and Amersham Typhoon NIR are variable-mode laser scanners that allow users to easily add or change filters to create new laser and filter combinations (Fig 2). • High resolution: resolve fine details in your sample with a pixel resolution of as low as 10 µm • High sample throughput: large scanning area of 40 × 46 cm enables you to simultaneously image up to 20 gels or blots, measuring 10 × 8 cm in size. It is also possible to scan up to 9 multiwell plates in a single scan. This throughput facilitates comparisons among blots and plates, reduces workload, and decreases waiting time. The IP model has a scanning area of 35 x 43 cm, which fits GE’s largest imaging plate Data file, 29229537AC Fig 2. Users can easily exchange the filters in Amersham Typhoon 5, RGB, NIR Plus, and NIR models. If a new filter is inserted or a filter is changed, the instrument automatically recognizes the filter and updat

Table 1. Typhoon scanner series comprises five different configurations Table 1. Typhoon scanner series comprises five different configurations Near-infrared fluorescence O X X X X Phosphor imaging Densitometry (OD) Amersham Typhoon IP X O Amersham Typhoon NIR O O Amersham Typhoon NIR Plus O O* Amersham Typhoon RGB X X Amersham Typhoon 5 X X OD = optical density * Optical density accessory (OD plate) is needed ** Only Green fluorescence channel is included All Amersham Typhoon models are versatile laser scanners for precise quantitation of biomolecules in gels,...

For the detection of radioactivity and fluorescence, emitted light is collected and transformed to an electrical signal by a photomultiplier tube (PMT). The electrical signal is then converted into digital information by A/D conversion for image display and analysis. Amersham Typhoon comes equipped with new bi-alkali and multi-alkali PMTs. This combination provides excellent detection over a very broad spectrum. Each PMT is selected for optimal response to the detected emission wavelength. The bi-alkali PMT is used for phosphor imaging, whereas the multialkali PMT is used for all fluorescence...

IRDye™ 680 goat anti-rabbit antibody Amersham WB 13.5% SDS-PAGE Excitation Emission filter 685 nm 720BP20 (IRshort) 3 pg 5.2 orders of magnitude R2=0.9988 and k=1.00 (trendline in log-log plot) Fig 7. Antibody conjugated with IRDye 680 was separated using an Amersham WB electrophoresis gel. To reduce noise, the gel was imaged with Amersham Typhoon using slow scan speed. A selection of a dilution series is shown in the image; the arrow indicates the limit of detection (LOD). The detection limit was 3 pg, and the linear dynamic range (DR) was 5.2 orders of magnitude The versatile Amersham Typhoon...

Sample CHO cell lysate Gel SDS-PAGE 8-18% Membrane Amersham Hybond™ P 0.45 Detection Primary antibodies Rabbit anti-ERK Mouse anti-GAPDH Secondary antibodies ECL Plex Cy3 GAR Alexa Plus 800 GAM Total protein stain Amersham QuickStain Imaging Excitation Emission Filter 532 nm Cy3 570BP20 (green) 685 nm IRshort 720BP20 (red) 785 nm IRlong 825BP30 (blue) Amersham Typhoon scanners are designed for use with analysis software such as Melanie™ 8 (Figs 14–16). The strengths of these imaging systems—high sensitivity and broad dynamic range for measuring low and high abundant proteins in one scan—make...

Benzoic acid-treated Fig 16. Representative Control (blue) and Treated (green) gel images of the experiment described in Fig 14 and Fig 15. Spots that are significantly upregulated (p values < 0.001) in the Treated group are shown in red; downregulated spots are shown in green. Fluorescence measurements of biological samples C autoradiographic standard (CFQ12000) 3 hour exposure to BAS-SR Imaging Plate Excitation Emission filter 635 nm IP BP390 0.00518 µCi/g 5.3 orders of magnitude R2=0.9998 and k=1.03 (trendline in log-log plot) 14 The large working area of Amersham Typhoon makes it ideally...