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GeneQuant 100 Spectrophotometer

GeneQuant 100 Spectrophotometer
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GE Healthcare Data File 28-9278-05 AA UV/Visible Spectrophotometers GeneQuant 100 Spectrophotometer GeneQuant™ 100 (Fig 1) is designed to measure the concentration and purity of nucleic acid and protein samples; and the density of bacterial cell cultures at a broad range of sample volumes. It can be used with a wide variety of cuvettes for sample volumes from 3 µl to 2 ml. GeneQuant 100 is preprogrammed with a range of preset methods for the convenient quantitation of proteins, nucleic acids, and bacterial cell cultures. In addition, absorbance and concentration measurements can be made at any wavelength providing flexibility for future applications. GeneQuant 100 Spectrophotometer delivers: • Convenience and ease of use: Calibration curves or ratio measurements are displayed at the touch of a button on the large, easy-to-use graphical display • Flexible analytical performance: You can save up to nine custom methods in a dedicated folder with an option for password protection. Visual inspection of a nucleic acid scan can quickly alert you to the presence of impurities—this is especially useful with RNA samples • Fast results: CCD array optics provide DNA scans in ~ 8 s and typical concentration measurements in < 5 s • Choice of data output: You can print to an integrated printer (optional) or to any suitable PC via a USB cable with Print via Computer (PVC) software (ordered separately) • Reliability and robust instrumentation: Press-to-read feature reduces the amount of time the lamp stays switched on. Optics with no moving parts reduces the incidence of optical misalignment Fig 1. GeneQuant 100 has a large, easy-to-read display which enables all your results to be viewed simultaneously. The key features of GeneQuant 100 are summarized in Table 1 below. Table 1. GeneQuant 100 key features Feature Wavelength range Wavelength calibration Stored methods Spectral bandwidth Wavelength accuracy Wavelength reproducibility Light source Detector Dimensions Weight Power requirements Specification 190-900 nm Automatic upon switch on 9 5 nm ± 2 nm ± 0.5 nm Long-life stabilized Xenon CCD Array 260 × 390 × 100 mm 4.5 kg 100–240 VAC ± 10%, 50/60 Hz, 50 VA

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Nucleic acids (Fig 2B) • Concentration and purity of nucleic acids • Choice of units: µg/ml, ng/µl, µg/µl, pmol/µl, and pmol • Correct for different cell pathlengths and dilution factors • UV spectrum (220 to 330 nm) analysis for hybridization, PCR, and sequencing studies or for quantitation of minipreps after isolation by chromatography Bacterial cell cultures • Measure OD600 prior to harvesting or induction Fig 2. GeneQuant 100 screenshots showing; (A) Simple interface for easy selection of applications; (B) A spectral scan of a nucleic acid sample; (C) Bradford calibration and (D)...

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GeneQuant 100 Spectrophotometer 2Pages

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GeneQuant 100 Spectrophotometer
2Pages