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GeneQuant 1300 Spectrophotometer

GeneQuant 1300 Spectrophotometer
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GE Healthcare Data File 28-9251-14 AA UV/Visible Spectrophotometers GeneQuant 1300 Spectrophotometer GeneQuant™ 1300 (Fig 1) is designed to measure the concentration and purity of nucleic acid and protein samples; and the density of bacterial cell cultures at a broad range of sample volumes. It can be used with a wide variety of cuvettes for sample volumes from 3 µl to 2 ml. GeneQuant 1300 is preprogrammed with a range of preset methods for the convenient quantitation of proteins, nucleic acids, and bacterial cell cultures. In addition, its wavelength scanning feature gives you the flexibility to design and store your own methods in a designated folder for easy access (Fig 2A). GeneQuant 1300 Spectrophotometer delivers: • Convenience and ease of use: Calibration curves, kinetics or ratio measurements are displayed at the touch of a button • Flexible analytical performance: You can save up to 90 custom methods in a dedicated folder with an option for password protection. In addition, you can perform a full wavelength scan from 200 to 900 nm in less than 5 s with zoom facility, peak identification, and on-peak confirmation. Visual inspection of a nucleic acid scan can quickly alert you to the presence of impurities—this is especially useful with RNA samples • Choice of data output: You can print to an integrated printer (optional) or to any suitable PC via a USB cable or wireless bluetooth accessory (optional) • Reliability and robust instrumentation: Press-to-read feature reduces the amount of time the lamp stays switched on. Optics with no moving parts reduces the incidence of optical misalignment Fig 1. GeneQuant 1300 has a large, easy-to-read display which enables all your results to be viewed simultaneously. The key features of GeneQuant 1300 are summarized in Table 1 below. Table 1. GeneQuant 1300 key features Wavelength range Wavelength calibration Stored methods Spectral bandwidth Wavelength accuracy Wavelength reproducibility Light source Detector Dimensions Weight Power requirements 190-1100 nm Automatic upon switch on 90 5 nm ± 2 nm ± 0.5 nm Long-life stabilized Xenon CCD Array 260 × 390 × 100 mm 4.5 kg 100–240 VAC ± 10%, 50/60 Hz, 50 VA

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Nucleic acids (Fig 2B) • Concentration and purity of nucleic acids • Choice of units: µg/ml, ng/µl, µg/µl, pmol/µl, and pmol • Correct for different cell pathlengths and dilution factors • UV spectrum (220 to 330 nm) analysis for hybridization, PCR, and sequencing studies or for quantitation of minipreps after isolation by chromatography • Characterize oligonucleotide primers (up to 66-mer) by conversion factor (µg/ml), molecular weight, theoretical absorbance (AU/µmol), and theoretical Tm Bacterial cell cultures • Measure OD600 prior to harvesting or induction Proteins (Fig 2C) • Methods...

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GeneQuant 1300 Spectrophotometer 2Pages

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GeneQuant 1300 Spectrophotometer
2Pages