GE Healthcare Data file 28-9610-74 AB Imaging systems, software, and accessories ImageQuant™ LAS 4000 biomolecular imager ImageQuant LAS 4000 (Fig 1) is a digital imaging system for sensitive, quantitative imaging of gels and blots, by chemiluminescence and fluorescence. A flexible combination of light sources and filters can be incorporated into the imager according to your needs. ImageQuant LAS 4010 is a model that is fully equipped with light sources for ultraviolet (UV) and visible (RGB) imaging. Both 4000 and 4010 models can be upgraded to perform infrared (IR) fluorescence imaging. ImageQuant LAS 4000 delivers: • Wide linear dynamic range: Capture and precisely quantitate weak and strong signals over four orders of magnitude in the same image, avoiding time-consuming multiple film exposures. • High resolution: Accurate quantitation of gels and films up to 21 × 14 cm in size. Up to 6.3 Mpixel image resolution is achieved via a unique diagonal pixel configuration. • Low noise: The camera is typically cooled to -25°C (maximum -35°C) enabling longer exposure times giving less background, which is especially important for precise quantitation of very weak signals in chemiluminescent Western blotting. • High sensitivity: ImageQuant LAS 4000 captures fluorescent and chemiluminescent signals on a Western blot down to picogram levels of target protein. Sensitivity may be increased by binning of up to 8 × 16 pixels. • Uniform quantitation: Distortion, dark frame, and flat frame corrections are applied to each imaging mode for optimal precision. • Multifluorescence detection: ImageQuant LAS 4010 extends functionality to image a wide range of UV- and RGB-excited labels and stains. Fig 1. ImageQuant LAS 4000 is a highly sensitive, CCD camera-based quantitative biomolecular imager. Description ImageQuant LAS 4000 is a sensitive imager for chemiluminescence, UV transillumination, and white epi-illumination imaging applications. ImageQuant LAS 4010 is additionally equipped for epifluorescent UV and RGB applications, as well as white transillumination. The system consists of an intelligent dark box, camera, lens, and automated five-position filter wheel with a standard filter for EtBr and open positions for the filters delivered with the optional fluorescent light sources. The system is simple to operate. Focusing, filters, illuminators, and exposure time are remotely controlled by a computer without having to open the cabinet. High resolution and precise quantitation of low signals are achieved using a multipurpose 16-bit, 3.2 Mpixel camera fitted with a large aperture F0.85 lens. The system has an automated iris, and responds rapidly when changing focus and settings. Chemiluminescent and colorimetric signals can be captured without changing the lens.

Technical features Sensitive chemiluminescence detection: The system is optimal for quantitation of chemiluminescent Western blots. F0.85 43 mm LAS high sensitivity lens F1.8 24 mm SIGMA™ wide view lens Gel documentation and quantitation: colorimetric-stained samples are easily imaged. Easily exchangeable light sources and filters: The system can image a wide range of fluorophores, and is easily adapted for use in multiple applications; filters and light sources are exchangeable in seconds. UV, red, green, blue, and IR light sources for epifluorescence as well as white light are available. Two...

Imaging applications ImageQuant LAS 4000 is a single platform for multiple imaging applications. The UV transilluminator for fluorescence detection of reagents such as EtBr (Fig 4), Deep Purple Total Protein Stain, and epi light sources for fluorescence detection of reagents such as SYPRO™ Rose and Qdot™ are provided as standard features. Quantitative Western blotting The high sensitivity of the system is designed to capture the chemiluminescent signals from Amersham™ ECL™, ECL Plus (Fig 3), and ECL Advance™ Western blotting reagents for quantitative purposes. Sample: Membrane: Detection: Transferrin...

Large gels Imaging of opaque stains such as Coomassie Blue, silver stain, and Amersham Rainbow™ markers and colorimetric Western blotting applications is achieved by standard epiwhite illumination or optional white transillumination (Fig 6). With the optional SIGMA F1.8 wide view lens, gels up to 25 × 25 cm can be imaged. Each imaging mode has individual settings for flat frame and dark frame corrections for uniform field imaging. Table 3. Optional filters for fluorescence Carbonic anhydrase 32 Sample: LMW marker Stain: Coomassie Blue Imaging: White transillumination LOD: 16 ng carbonic anhydrase...

Imager performance Chemiluminescence Amersham ECL Amersham ECL Plus Amersham ECL Advance Fluorescence via UV transilluminator (312 nm) EtBr + + Deep Purple + + SYBR Green I & II + + SYBR Gold + + Fluorescence via UV epi-illuminator (365 nm) EtBr + SYPRO Rose + Qdot 605, 655, 705, 800 + Fluorescence via blue epi-illuminator (460 nm) Alexa Fluor™ 488 + Cy2 + SYBR™ Green I & II + SYBR Gold + SYPRO Ruby + SYPRO Orange + FITC + FAM™ + EGFP + ECFP + AttoPhos™ + ECL Plus + Pro-Q™ Emerald 488 + Fluorescence via green epi-illuminator (520 nm) SYPRO Red + Cy3 + TAMRA™ + 5-ROX™ + HEX™ + Alexa Fluor 532,...

Ordering information System Upgrades and accessories Code no. * For chemiluminescence, UV transillumination, and white epi-illumination imaging applications. Includes intelligent dark box, camera head, F0.85 43 mm LAS high sensitivity lens, calibration plates DI and FL (green), AC cable (EU and USA), USB cable, operation manual, auto filter changer, UV transmitted light source, white epi-light source, EtBr detection filter, EPI tray, UV transmission tray, and 10 gel sheets. White light set, 1 pair F1.8 24 mm SIGMA wide view lens and F-mount adapter UV transmission tray and 10 gel sheets Pack...