GE Healthcare Data file 28-9301-69 AC UV/Visible spectrophotometers NanoVue™ Plus Spectrophotometer NanoVue Plus Spectrophotometer (Fig 1) is a stand-alone instrument with a novel drop-and-measure sample plate that can be used with a wide range of chemicals. NanoVue Plus can be used for the accurate and reliable quantitiation of nucleic acids and protein samples. The ability to pipette samples of 0.5 to 5 μl directly onto the sample plate eliminates the need for cuvettes or other sample devices. After measurement, the sample can either be easily recovered with a pipette or quickly discarded by wiping the sample plate clean for the next measurement. NanoVue Plus is pre-programmed with a range of preset methods for the quantitation of nucleic acids and proteins, using UV or dye-intercalation methods (Lowry, Bradford, BCA, and Biuret). In addition, wavelength scanning gives you the flexibility to design your own methods, which can be stored in a personal folder for easy access. The instrument does not require a computer because it has a direct user interface. Fast instrument start-up with automatic selfcalibration means NanoVue Plus Spectrophotometer is always ready to use. NanoVue Plus Spectrophotometer offers: • Novel gold-colored hydrophobic sample plate: Save time and effort by directly pipetting your sample onto the sample plate for measurement. The hydrophobic coating facilitates sample recovery and minimizes the potential for contamination of the sample mechanism thus improving data precision (Fig 2). The plate is compatible with a wide range of chemicals including DMSO and DMF • Low sample volumes: Reduce sample loss and eliminate the need for dilution by using low volumes of 0.5 to 5 μl for sample measurement. The appropriate pathlength for your sample volume is selected automatically unless you opt for manual selection • Fast measurements: Read-time is typically < 5 s per sample and you save more time by not having to wash cuvettes or dilute samples Fig 1. NanoVue Plus Spectrophotometer uses a novel drop-and-measure sample plate which eliminates the need for cuvettes. • Convenience and ease of use: Large, high-resolution graphical display enables a quick read of relevant results. Calibration curves, kinetics or ratio measurements are all displayed at the touch of a button (Fig 3) • Flexible analytical performance: Full wavelength scan in less than 5 s from 200 to 900 nm with zoom facility, peak identification, and on-peak confirmation. Visualization of nucleic acid scans allows impurities to be detected and this is especially useful with RNA samples • Choice of data output: Print to an integrated printer (optional) or to any suitable PC via a USB, wireless (Bluetooth) connection or SD card • Reliability and robust instrumentation: Press-to-read feature reduces the amount of time the lamp stays switched on. Optics with no moving parts minimizes the incidence of optical misalignment

BSA carry over data (mg/ml) Nucleic acids NanoVue Plus Spectrophotometer allows you to measure the concentration and purity of nucleic acids in a variety of units (μg/ml, ng/μl, μg/μl, pmol/μl, and pmol) and also, to correct for dilution factors where necessary. Sample number water pre BSA sample water post BSA sample Fig 2. Sample carry over study using 25 mg/ml BSA with successive additions of a water-protein-water cycle of samples, which was repeated 25 times. The data showed no significant change in absorbance readings during the study for water sample readings or BSA samples readings thus...

Protein concentration can be determined in the near UV at 280 nm due to absorption by tyrosine, tryptophan, and phenylalanine amino acids. The absorbance at 280 nm varies greatly for different proteins because of their amino acid content therefore, the specific absorption value for a particular protein must be determined. NanoVue Plus has in-built extinction coefficients for BSA, IgG, and lysozyme thus allowing you to select the most appropriate for your protein sample or you can input your own extinction coefficients. Typical results for BSA and IgG values using the absorbance at 280 nm program...

Drop. Measure. Done. Ordering information NanoVue Plus provides intelligent performance across all spectrophotometry applications (Fig 7). 2. Lower the sample plate head 3. Read your result NanoVue Plus with integrated printer (US & CA only) NanoVue Plus with integrated printer (Worldwide except US & CA) NanoVue Plus, print via computer (US & CA only) NanoVue Plus, print via computer (Worldwide except US & CA) 1. Just pipette your sample onto the sample NanoVue Plus with wireless connectivity (US & CA only) 28-9569-64 Fig 7. Operation of NanoVue Plus Spectrophotometer. Summary NanoVue Plus Spectrophotometer...