New generation Size Exclusion Chromatography (SEC) columns

Product catalog summary

Introduction
The document introduces the new generation of size exclusion chromatography (SEC) columns called "Increase" by GE Healthcare. These columns are designed for high-resolution small-scale preparative purification and analysis, accommodating sample volumes up to 500 µL. They offer improved performance over predecessor columns, including reduced run time, higher resolution, and better lot-to-lot consistency.

Specifications
The new generation SEC columns include:

Superdex 30 Increase: For peptides and small biomolecules, with a fractionation range of Mr ~ 100 to 7000.
Superdex 75 Increase: For recombinant tagged proteins, with a fractionation range of Mr ~ 3000 to 70,000.
Superdex 200 Increase: For monoclonal antibodies and other antibodies, with a fractionation range of Mr ~ 10,000 to 600,000.
Superose 6 Increase: For larger proteins and protein complexes, with a fractionation range of Mr ~ 5000 to 5,000,000.

Performance Improvements
The new columns deliver up to 50% higher resolution at the same flow rate or three times faster separation with the same resolution compared to previous models. The agarose resins have been optimized with smaller, more rigid beads and a narrower particle size distribution for higher selectivity.

Column Dimensions and Applications
Three column dimensions are available to fit different needs:

10 mm × 300 mm: Suitable for high-resolution analysis and small-scale preparative runs with sample volumes of 25 to 500 µL.
3.2 mm × 300 mm: Designed for high-resolution analysis with sample volumes of 4 to 50 µL, offering low sample and buffer consumption.
5 mm × 150 mm: Ideal for purity checks and rapid screening with low sample and buffer consumption.

Ordering Information
The document provides specific ordering codes for each column dimension and type, emphasizing compatibility with ÄKTA pure 25 systems and traditional HPLC systems.

Conclusion
The new generation SEC columns by GE Healthcare offer significant advancements in resolution, speed, and consistency for small-scale preparative purification and analysis, making them a valuable tool for researchers and laboratories.

Catalog excerpts

New generation Size Exclusion Chromatography (SEC) columns-1

New generation Size Exclusion Chromatography (SEC) columns Maximized resolution and optimized run time for small-scale preparative purification and analysis Our new generation size exclusion chromatography columns called “Increase” are designed for high resolution in small-scale preparative purification and analysis for sample volumes up to 500 µL. They deliver improved performance compared to predecessor columns*: • Reduced run time with maintained resolution for time saving or increased throughput • Higher resolution for improved protein purity and analysis data • Higher lot-to-lot consistency for better reproducibility In 2017, GE expands the range of new generation SEC columns with Superdex™ 30 Increase • Four choices of new generation SEC resins for small-scale preparative purification and analysis • Superdex 30 Increase is the replacement for Superdex Peptide columns Peptides and other small biomolecules Fractionation range Mr ~ 100 to 7000 Recombinant tagged proteins Fractionation range Mr ~ 3000 to 70 000 mAb and other antibodies Fractionation range Mr ~ 10 000 to 600 000 Larger proteins and protein complexes† Fractionation ra

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New generation Size Exclusion Chromatography (SEC) columns-2

New generation SEC columns deliver improved performance compared to predecessor columns* The agarose resins have been optimized to deliver improved performance compared with their predecessors (smaller and more rigid beads, narrower particle size distribution, higher selectivity). Higher resolution at the same flow rate 800 Flow rate: 0.5 mL/min 0.5 mL/min Flow rate: Superdex™ Peptide 10/300 GL 10/300 400 Superdex™ Peptide GL Superdex 30 Superdex10/300 GL 10/300 GL Increase 30 Increase 350 Superdex Superdex 250 Peptide 30 Increase 10 20 20 30 30 40 Retention time (min) time (min) Retention Superdex...

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