SimpliNano spectrophotometer
4Pages

UV/Vis spectrophotometers SimpliNano™ spectrophotometer SimpliNano is a simple-to-use microvolume spectrophotometer for straightforward concentration and purity measurements of nucleic acids and proteins (Fig 1). SimpliNano uses a built-in sample port that can be used with a variety of common lab chemicals and has no moving parts, making it reliable, easy-to-use, and clean. It has a fixed pathlength that does not require any calibration. The ability to pipette volumes down to 1 µl directly onto the sample port eliminates the need for cuvettes or other sampling tools. After measurement, the sample can either be easily recovered with a pipette or quickly discarded by wiping the sample port clean for the next measurement. SimpliNano is preprogrammed with a range of preset methods for the quantitation of nucleic acids and direct UV proteins, with graphical display of resulting wavescans. The SimpliNano direct user interface means that there is no need for a computer, so fast instrument start-up is possible. SimpliNano offers the following benefits: • Simple sample handling: Built-in sample port (Fig 2) with one fixed pathlength means that samples can be added and removed without the need for time-consuming pathlength calibration. SimpliNano contains no moving parts, which contributes to excellent instrument reproducibility and low maintenance. • Low sample volumes: Reduce sample loss and eliminate the need for dilution by using low volumes of 1 to 5 μl for sample measurement. The sample can either be recovered or simply wiped away after measurement. • Built-in life science methods: Measures nucleic acid concentrations at 260 nm, protein concentrations at 280 nm, and provides 230/260 and 260/280 ratios for determining sample purity. Wavescan plots from 220 nm to 330 nm can be achieved for nucleic acids and from Fig 1. SimpliNano is an easy-to-use, low-maintenance spectrophotometer designed for analysis of low volumes of nucleic acid and protein samples, shown here with printer option. 250 nm to 350 nm for protein. The methods include options for background correction at 320 nm and 340 nm. • Flexible instrument control: Use as either a standalone instrument using its large, built-in screen for quick measurements or operate from a PC with optional Datrys software. • Choice of data output: Output to any suitable PC via a USB cable, to an optional integrated printer, or to a USB stick. Technical specifications for SimpliNano are given in Table 1.

Table 1. Technical specifications for SimpliNano Fig 2. The built-in sample port allows direct application of 1 to 5 µl volumes of sample. SimpliNano mean result Linear (nominal line) Fig 3. DNA concentration curve (4 to 2500 μg/ml) generated from 10 replicate salmon sperm dsDNA samples (2 µl volumes) measured at 260 nm on SimpliNano. The data in the graph is representative data from one instrument used in the validation study where a total of 6 instruments were tested. Wavelength range Minimum sample size Absorbance range -0.3 to 2.5 Abs. units (10 mm equivalent to 50 Abs. units) Spectral...

Sample can be simply removed with a tissue to prevent cross-contamination thus improving data precision. To demonstrate this, sample carryover was determined using high concentrations of protein (BSA) and the results of the carryover experiments are shown in Figure 5. Quantity Code number 29-0617-11 SimpliNano with printer (North America only) SimpliNano with printer (Worldwide except US and Canada) SimpliNano (Worldwide except US and Canada) SimpliNano (North America only) BSA A280 absorbance (background corrected, normalized to 10 mm) Ordering information Related products Quantity Code...

GE and GE monogram are trademarks of General Electric Company. For local office contact information, visit www.gelifesciences.com/contact www.gelifesciences.com/spectros GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire, HP7 9NA UK GenomiPhi, GFX, GraviTrap, illustra, NanoVue, SimpliNano, and SpinTrap are trademarks of General Electric Company or one of its subsidiaries. GenomiPhi: For use only as licensed by Qiagen GmbH. and GE Healthcare. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent...