Typhoon FLA 7000 biomolecular imager
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GE Healthcare Life Sciences Data file 28-9610-73 AC Imaging systems, software, and accessories Typhoon™ FLA 7000 and 7000 IP biomolecular imager Typhoon FLA 7000 (Fig 1) is a fast and versatile laser scanner for biomolecular imaging applications including sensitive and quantitative measurements of radioisotopic labels by storage phosphor and single fluorescence, as well as digitization of colorimetric stains (e.g., Coomassie™ Blue and silver-stained gels). Typhoon FLA 7000 IP is a model dedicated to phosphor imaging. It comes with only one laser configuration. Upgrade paths for any of blue, green, and red excited fluorescence imaging are provided. Typhoon FLA 7000 delivers: • Versatility: the system images radioisotopic, visible fluorescent, chemifluorescent, and colorimetric samples. • High resolution and quantitation: 16-bit images are generated at up to 25 µm pixel resolution. A linear signal response over a span of five orders of magnitude gives precise quantitation in gels, blots, and tissue sections. • High speed: a 24 × 25 cm gel can be scanned in less than two minutes at 100 µm resolution without compromising sensitivity. • High sample throughput: a scanning area of 24 × 40 cm enables simultaneous imaging of up to 12 gels or blots, measuring 10 × 8 cm in size. This facilitates comparisons among blots, and reduces workload and waiting time. Typhoon FLA 7000 is a nonconfocal variable mode laser scanner offering high speed and resolution for precise quantitation of proteins, nucleic acids, and other biomolecules. Four lasers are preinstalled in the system: blue (473 nm), green (532 nm), red (635 nm), and red (650 nm). The system can image a sample measuring 24 × 40 cm in 2.5 min, offering high throughput for the multiuser lab environment. Fig 1. Typhoon FLA 7000 biomolecular imager provides fast, sensitive imaging, and high versatility. The system provides several imaging modes, such as fluorescence, filmless autoradiography, and digitization of colorimetrically stained gels (e.g., Coomassie Blue and silver stain). Since the system is fast and includes predefined methods, Typhoon FLA 7000 is well-designed for meeting the various imaging demands of different applications. Applications include phosphorimaging of radioisotopes, and fluorescence detection, such as 1-D and 2-D gels poststained with Deep Purple™, SYPRO™ Ruby or SYPRO Red, and DNA imaging by SYBR™ Green or Cy™5. Fig 2. Filters are easily exchanged by the user.

Table 1. Typhoon FLA 7000 specifications Technical features Imaging modes: Excitation wavelengths: Radioisotopes: Dynamic range: Bit depth: Max scanning area: Pixel sizes: Standard filters: Dimensions (W × H × D): Weight: Line frequency: Temperature: Humidity: Supply voltage: Power consumption: Fast, simple, and high quality imaging Fluorescence, phosphorimaging, and digitization 473 nm (blue LD laser), 532 nm (green SHG laser), 635 nm (red LD laser), and 650 nm (red LD laser) 3 H, 11C, 14C, 125I, 18F, 32P, 33P, 35S, 99mTc, and other sources of ionizing radiation 5 orders of magnitude...

Image analysis For the detection of radioactivity, fluorescence and chemifluorescence, emitted light is collected and transformed to an electrical signal by a photomultiplier tube (PMT). The electrical signal is then converted into digital information by A/D conversion for image display and analysis. Designed for seamless data transfer and quantitative gel and blot analysis, we provide image analysis software for use with Typhoon FLA 7000 (Table 4). 1-D gel electrophoresis, dot blots, arrays, user-defined gel analysis Upon excitation, light is emitted from a fluorescently labeled sample in...

Carbonic anhydrase Sample: Gel: Label: Imaging: Cy3: LOD: L: DR: Carbonic anhydrase Carbonic anhydrase 12% acrylamide Tris-glycine CyDye™ DIGE fluor, Cy3 minimal dye Excitation Emission filter 532 nm O580 0.4 ng carbonic anhydrase R2=0.999 2.7 orders of magnitude Fig 6. Different concentrations of carbonic anhydrase were labeled with CyDye DIGE fluor, Cy3 minimal dye and subjected to 1-D electrophoresis. The gel was imaged with Typhoon FLA 7000. The LOD was 0.4 ng carbonic anhydrase and the linear DR was 2.7 orders of magnitude. Arrow indicates the LOD. Carbonic anhydrase 12% acrylamide...

Ordering information System Quantity Code number * Includes 473 nm, 532 nm, 635 nm and 650 nm lasers, bialkali PMT, filter holder, IP filter, Y520 filter, O580 filter, R670 filter, Fluor Stage, Membrane Weight, Phosphor Stage, Fluorescent plate for digitization, capture software, USB cable, mains cables (EU and USA), User manual, Getting Started Guide, and Control Software User Manual. One license of ImageQuantTL software is provided with Typhoon FLA 7000 and 7000 IP ** Includes 650 nm laser, bialkali PMT, filter holder, IP filter, Phosphor Stage, capture software, USB cable, mains cables...

GE, imagination at work, and GE monogram are trademarks of General Electric Company. Amersham, Cy, CyDye, Deep Purple, ECL, Hybond, ImageMaster, ImageQuant, and Typhoon are trademarks of GE Healthcare companies. Deep Purple Total Protein Stain: Deep Purple Total Protein Stain is exclusively licensed to GE Healthcare from Fluorotechnics Pty Ltd. Deep Purple Total Protein Stain may only be used for applications in life science research. Deep Purple is covered under a granted patent in New Zealand entitled “Fluorescent Compounds”, patent number 522291 and equivalent patents and patent...