WAVE Bioreactor 200 System
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GE Healthcare Life Sciences Data file 29-0237-13 AA WAVE Bioreactor™ 200 system WAVE Bioreactor 200 system (Fig 1) is part of GE Healthcare Life Sciences' ReadyToProcess platform of ready-to-use products. The system is a cell culture device for the production of recombinant proteins in mammalian and insect cell lines in batch, fed-batch, and perfusion culture. Culture medium and cells are loaded into a single-use, presterilized bag known as the Cellbag™ bioreactor. The Cellbag bioreactor is placed on an electric rocking base. The rocking motion of this base induces waves in the cell culture fluid within the Cellbag bioreactor to provide efficient mixing and gas transfer. The resulting environment within the Cellbag bioreactor can easily support 1 × 107 cells/mL. The Cellbag bioreactor requires no cleaning or sterilization, providing easy operation and protection against cross-contamination. As part of ReadyToProcess platform, the WAVE Bioreactor brings flexibility and speed to upstream and downstream processing of biologicals. The product range comprises WAVE Bioreactor systems, WAVE Mixer™, tubing sealers and fusers, hollow fiber and normal flow filters, prepacked chromatography columns, and ÄKTA™ ready chromatography system with a disposable flow path, as well as the assemblies and connections in between. The platform is scalable from the lab bench to manufacturing. WAVE Bioreactor 200 system delivers: • Convenience: Presterilized, single-use Cellbag bioreactors protect against the risk of cross-contamination, require no cleaning, and involve minimal validation and they are supplied in a ready-to-use format • Reliability: Cellbag bioreactors, including all fittings and filters, are supplied sterile and ready for use. They are suitable for cGMP commercial production and a biosafety cabinet is not required for inoculation or sampling • Flexibility: Multiple instrument configurations for suspension, microcarrier, batch, fed-batch, or perfusion culture • Versatility: The 200 system is capable of handling culture volumes from 10 to 100 L Fig 1. The WAVE Bioreactor 200 system is suitable for culture volumes of 10 to 100 L. System descriptions The WAVE Bioreactor 200 system comprises integral rocking, temperature, weight, and airflow controllers. The selfcontained system is designed for use with working culture volumes of 10 to 100 L in applications such as inoculum scale-up, R & D, commercial production, vaccine production, and antibody manufacture. For additional flexibility, optional modules including DO, CO2, O2, weight/perfusion, and pH control can be added while builtin Ethernet and MODBUS data ports allow communication with other software. Applications The WAVE Bioreactor system is suitable for use with anchorage-dependent cells in addition to cell suspensions and has applications in: • Monoclonal antibodies • Insect cell culture • Virus production • Growing pathogens or other high-containment systems • Inoculum scale-up • Protein expression • Primary cell line expansion

UNICORN DAQ 1.0 software facilitates real time data acquisition for the management and evaluation of results from cell cultures performed using up to four different WAVE Bioreactor systems connected to a single PC. The WAVE Bioreactor system can be connected directly or networked to the software providing a common platform and user interface for monitoring and storing result data. A dynamic graphical user interface informs you about the real-time status of the run being monitored. During a run, data is automatically saved to a local hard drive or server in a secure and unalterable result...

Technical information and specifications CO2/air mix controller The CO2/air mix controller connects to a supply of 100% CO2 to provide CO2/air concentrations between 0% and 15% CO2. The instrument is useful for pH control of bicarbonate buffered cell culture media. WAVE Bioreactor 200 system Features Touch panel operator interface Direct drive electronic linear motor Temperature control Adjustable rocking rate from 4 to 25 rocks/min with acceleration control Temperature is regulated for single and dual WAVE Bioreactor systems (Fig 4). Adjustable rocking angle from 2° to 9° Integral...

Ordering information Product_Code number Related literature Disposable Cellbag bioreactors for the WAVE Bioreactor system, Data file 28-9511-36 ReadyToProcess connectivity, Data file 29-0138-84 WAVEPOD™ II Integrated Controller, Data file 28-9606-57 WAVE Bioreactor 2/10 and 20/50 systems, Data file 28-9520-58 WAVE Bioreactor 500/1000 system, Data file 29-0237-12 GE, imagination at work, and GE monogram are trademarks ot General Electric Company. AKTA, Cellbag, ReadyToProcess, UNICORN, WAVE Bioreactor WAVE Mixer, and WAVEPOD are trademarks of GE Healthcare For local office contact...