GE Healthcare Life Sciences Data file 29-0237-12 AA WAVE Bioreactor™ 500/1000 system WAVE Bioreactor 500/1000 system (Fig 1) is part of GE Healthcare Life Sciences' ReadyToProcess platform of ready-to-use products. The system is a cell culture device for the production of recombinant proteins in mammalian and insect cell lines in batch, fed-batch, and perfusion culture. Culture medium and cells are loaded into a singleuse, presterilized bag known as the Cellbag™ bioreactor. The Cellbag bioreactor is placed on an electric rocking base. The rocking motion of this base induces waves in the cell culture fluid within the Cellbag bioreactor to provide efficient mixing and gas transfer. The resulting environment within the Cellbag bioreactor can easily support 1 × 107 cells/mL. The Cellbag bioreactor requires no cleaning or sterilization, providing easy operation and protection against cross-contamination. As part of ReadyToProcess platform, the WAVE Bioreactor brings flexibility and speed to upstream and downstream processing of biologicals. The product range comprises WAVE Bioreactor systems, WAVE Mixer™, tubing sealers and fusers, hollow fiber and normal flow filters, prepacked chromatography columns, and ÄKTA™ ready chromatography system with a disposable flow path, as well as the assemblies and connections in between. The platform is scalable from the lab bench to manufacturing. WAVE Bioreactor 500/1000 system delivers: • Convenience: Presterilized, single-use Cellbag bioreactors protect against the risk of cross-contamination, require no cleaning, and involve minimal validation and they are supplied in a ready-to-use format • Reliability: Cellbag bioreactors, including all fittings and filters, are supplied sterile and ready for use. They are suitable for cGMP commercial production and a biosafety cabinet is not required for inoculation or sampling Fig 1. The WAVE Bioreactor 500/1000 system is suitable for culture volumes of 50 to 500 L. System descriptions The WAVE Bioreactor 500/1000 system comprises integral rocking, temperature, weight, and airflow controllers. The self-contained system is designed for use with working culture volumes of between 50 and 500 L in applications such as inoculum scale-up, R & D, process development, commercial production, vaccine production, and antibody manufacture. For additional flexibility, optional modules including DO, CO2, O2, weight/perfusion, and pH control can be added while builtin Ethernet and MODBUS data ports allow communication with other software. Applications The WAVE Bioreactor system is suitable for use with anchorage-dependent cells in addition to cell suspensions and has applications in: • Flexibility: Multiple instrument configurations for suspension, microcarrier, batch, fed-batch, or perfusion culture • Monoclonal antibodies • Versatility: The 500/1000 system is capable of handling culture volumes from 50 to 500 L • Virus production • Insect cell culture • Growing pathogens or other high-containment systems • Inoculum scale-up • Protein expression • Primary cell line expansion

Rapid release Cam-lock levers secure the Cellbag bioreactor in place on the rocking platform allowing bags to be attached and removed in minutes. The holder design ensures that the Cellbag bioreactor is locked in the optimal position for oxygen transfer and mixing. The color touchscreen (Fig 2) located on the control panel of the WAVE Bioreactor 500/1000 system enables the setup, control, and viewing of all cell culture parameters while data can be monitored graphically in real time. The main menu provides an overview of key operating conditions and it is the main access screen for all controls....

Technical information and specifications Ordering information WAVE Bioreactor 500/1000 system Direct drive electronic linear motor Adjustable rocking rate from 4 to 25 rocks/min with acceleration control Adjustable rocking angle from 0.5° to 4° Integral temperature controller with heater Integral weight controller Touch panel operator interface Integral airflow controller Integral PID controller for automatic temperature, O2, CO2, and pH adjustment Code number Real-time data monitoring Related literature RS-485 MODBUS communications port Disposable Cellbag bioreactors for the WAVE Bioreactor...

GE, imagination at work, and GE monogram are trademarks of General Electric Company. ÄKTA, Cellbag, ReadyToProcess, WAVE Bioreactor, WAVE Mixer, and WAVEPOD are trademarks of GE Healthcare companies. For local office contact information, visit www.gelifesciences.com/contact www.gelifesciences.com/readytoprocess GE Healthcare Bio-Sciences AB Björkgatan 30 751 84 Uppsala Sweden © 2012 General Electric Company – All rights reserved. First published Jun. 2012 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy...