Swab:Direct™ extraction kit is designed to perform PCR test from nasopharyngeal and oropharyngeal swab samples without prior DNA/RNA purification process. The buffer includes high concentrated alkaline compounds for lysis and elution of sample organisms. This product is easy to use by just mixing the swab sample and extraction buffer without additional processes for preparing nucleic acid template to run PCR tests. Fast and simple method to extract DNA – Whole process in 5 minutes Minimized hands-on time Ideal for on-site testing Room temperature storage PROTOCOL Shake the tube up and down for 5-10 times and incubata it in the room temperature for 5 minutes for lysis Put the swab stick in the tube of Swab:Direct™ lysis buffer Snap off the swab stick leaving the swab tip into the tube, and close the cap. Take 10 μL of supernatant of the mixture and use this as template of PCR test Extraction Buffer

Media:Direct™ Extraction Buffer COMPARISON OF NUCLEIC ACID EXTRACTION PERFORMANCE 1) PCR result using nucleic acid extracted by Swab:Direct™ Extraction Kit Sample Type Ct. value of repeated tests 1 Nasopharyngeal swab Oropharyngeal Swab 2) PCR result using nucleic acid extracted by Spin Column Method from A Company Sample Type Ct. value of repeated tests 1 Nasopharyngeal swab Oropharyngeal Swab 3) PCR result using nucleic acid extracted by Magnetic Bead Method from B Company Sample Type Ct. value of repeated tests 1 Nasopharyngeal swab Oropharyngeal Swab Real-time PCR System: GENECHECKER® UF-300...