GenSolve™ DNA Recovery Kit More and Better DNA Paper is a fast and easy way to collect DNA from a variety of biological samples. Blood, buccal cells, plants, bacteria and many other sample types are applied to cards where the cells are instantly lysed and DNA is captured in the cellulose matrix. After drying, the samples are stable and ready for long term storage. GenSolve recovers the same high quality double stranded DNA from all papers, standard filter paper, GenSaver, GenCollect, or Guthrie cards, and the process is fast and easy. Any possible downstream interference by the FTA chemicals is eliminated as they are removed when using GenSolve. GenSolve also delivers the highest yields of DNA when compared to other generic extraction protocols. GenSolve is a robust kit for extracting high quality, high yield double-stranded DNA preserved in GenPlates®, GenSaver™ cards, FTA paper, GenCollect™ cards. Guthrie cards and all filter papers. Existing generic methods of extracting DNA from the cellulose matrix include alkaline conditions, and high heat, both of which result in the recovery of single-stranded DNA; restriction digest or organic extractions yield doublestranded DNA but are labor intensive. • High yields of double stranded DNA • >35 Kb molecular weight DNA • Fast, only requires 90 minutes • Simple easy process Fast Simple Protocol The GenSolve procedure consists of incubating 6 mm FTA discs at 65˚C with a protease combined with a proprietary high pH solution and 1% lithium dodecyl sulfate. The incubation releases DNA, proteins and cellular debris from the matrix in a highly efficient manner. After centrifugation the eluate can be purified by a standard bind-washelute method for DNA isolation and concentration. The entire process from punch to purified double stranded DNA takes less than 90 minutes. From filter paper to purification in only 90 minutes FTA OR FILTER PAPER DISC Continue with DNA Purification EXTRACTION Recovery of high quality double stranded DNA using GenS

GenSolve Performance Yield of DNA: GenSolve recovers a significant amount of the DNA trapped within the matrix when whole blood is spotted to the FTA Cards. Yields are typically between 50 - 360 ng from 10 |jL of whole blood. Yield varies depending on the white blood cell count of the initial sample and the normal variability between donors. Reproducibility: GenSolve is reproducible with an average CV of 11%. Over 90 discs containing 10 jL of whole blood from 30 unique samples were processed by the GenSolve method in triplicate. The % CV was calculated from the standard deviation for each triplicate. High...