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GenTegra® -DNA Active Chemical Protection™

GenTegra® -DNA Active Chemical Protection™

GenTegra® -DNA Active Chemical Protection™

Product catalog summary
Introduction
GenTegra™ DNA offers a solution for protecting DNA samples from hydrolysis and oxidation, ensuring long-term stability at ambient temperatures. This product provides an alternative to freezing, which only slows degradation processes.

Specifications
GenTegra DNA is available in various formats, including screw cap tubes, cluster tubes, microtubes, and microtiter plates. It supports DNA application amounts ranging from 0.05 µg to ≤20 µg and sample volumes from 1-250 µl. The product is designed to withstand extreme temperatures from -80 °C to 76 °C, exceeding military and Federal Express® specifications.

Stability and Protection
DNA samples stored with GenTegra DNA show no degradation after the equivalent of 16 years at ambient temperature. Accelerated stability studies confirm protection without visible degradation. Ongoing experiments demonstrate quality retention over 4.5 years, with samples performing identically in downstream applications.

Recovery and Applications
Recovering DNA samples is straightforward; adding water restores 100% of the sample for use in various applications such as transfections, quantitative PCR, gene expression, next-generation sequencing, and forensics. The product ensures over 99% recovery efficiency.

Quality Retention
Amplification of mitochondrial DNA in qPCR indicates intact DNA up to 2.5kb after 4.5 years, with normal cycle thresholds. Long-range PCR confirms the integrity of stored DNA samples.

Conclusion
GenTegra DNA provides a reliable method for stabilizing and protecting DNA samples during storage and transport, offering significant advantages over traditional freezing methods.
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Catalog excerpts

GenTegra® -DNA Active Chemical Protection™-1

Don’t just store your DNA samples, protect them GenTegra™ DNA protects DNA from hydrolysis and oxidation, stabilizing it for long-term ambient temperature storage, while freezing merely slows these processes. GenTegra DNA is like insurance for storing and shipping your samples, because it stabilizes them for a wide temperature range. Dry ice and cold packs cannot protect your samples during delivery delays, and even a few extra days can cause sample loss. When shipped using GenTegra DNA, your sample is completely protected even after days or weeks of delay. You might even save money on shipping costs and rush charges. Recovering your sample after storage or shipment is easy. Simply add water to recover 100% of your sample, and it is ready for any downstream application. Start protecting your samples the better way, using GenTegra DNA. Long Term Protection and Stability DNA samples stored on GenTegra DNA show no degradation after the equivalent of 16 years storage at ambient temperature. Accelerated stability studies show DNA sample protection with no visible degradation.1 250 ng/lane genomic DNA stored on GenTegra DNA for six months at ambient (25 °C) and elevated temperatures, Ongoing ambient temperature experiments show retention of sample quality at 4.5 years After an initial six month incubation period at 25 °C, 37 °C, and 56 °C, samples stored on GenTegra DNA were stored at ambient temperature (25 °C) for four years, duplicating actual storage conditions. These samples show no degradation and perform identically in all downstream applications. 25 °c 37 “C 56 °c Temperature at 7mo 7 mo 7mo initial storage date Bruskov, VI. Malakhova, LV. Masalimov, ZK. Chernikov, AV. (2002) Heat-induced formation of reactive oxygen species and 8-oxoguanine, a biomarker of damage to DNA. Nucleic Acids Research, 6, 1354-1363.

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GenTegra® -DNA Active Chemical Protection™-2

• Next-generation sequencing • Genotyping • HLA typing • Forensics Quality Retention Amplification of mitochondrial DNA in qPCR shows that the 4.5 year old DNA is intact to at least 2.5kb. Ct was normal (19-23 cycles) for all 4.5 year old samples. Downstream applications supported: • Transfections • Quantitative PCR • Gene expression • RRN profiling For more info visit our website: Stabilize in GenTegra DNA and dry *barcode optional Toll free: 844.540.4000 • Tel: 925.461.3010 • Fax: 925.461.3086 • www.GenTegra.com Copyright© 2014 GenTegra LLC.. All rights reserved. Gentegra, Gentegra design,...

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