Sample Prep Solution 3-MCPD Efficient Sample Preparation for the determination of 3-MCPD, 2-MCPD and Glycidyl in edible oil and fat Automated system for ISO 18363-1, AOCS Cd 29c-13, and DGF C-VI 18 (10) High stability and lowest limits of determination achieved using automated evaporation step

Sample Prep Solution 3-MCPD During edible oil refining processes, 2-MCPD, 3-MCPD and glycidyl fatty acid esters can be generated, resulting in a contaminated product. The GERSTEL 3-MCPD Sample Prep Solution enables automated determination of these potentially health relevant contaminants supporting the methods ISO 18363-1, AOCS Cd 29c-13, and DGF C-VI 18 (10). In fats and oils, glycerol is contained in the form of fatty acid esters (triglycerides). Since many oils are not suitable for consumption and are not stable in storage in their native, untreated form, they are refined to remove unwanted...

Weigh a 100 mg sample into a vial Fill a second vial with sodium sulfate drying agent (drying vial) - optional Add MTBE to the sample Add ISTD solution and mix, or melt and mix (solids) Add MeOH/NaOH mixture Agitate and incubate Add acidic NaCl solution (Assay A) Add acidic NaBr solution (Assay B) Add n-hexane for matrix extraction Agitate and incubate Discard hexane phase Repeat extraction with n-hexane twice Perform multiple analyte extractions using MTBE/Ethylacetate 3:2 (v/v), transfer the organic phases to the drying vial Add phenylboronic acid solution Evaporate to dryness and derivatize...

a.qSTLL Appteeter GERSTEL AppNote 191 [1] offers a detailed overview of the performance of the GERSTEL 3-MCPD Sample Prep Solution. Among other things, it is demonstrated that results achieved using the automated sample preparation procedure correlate well with results from established manual sample preparation procedures. The usefulness for different types of oil is discussed, and linearity and repeatability shown. All results demonstrated the suitability of the automated Sample Prep Solution for routine analysis. [1] Lucas, D.; Hoffmann, A.; Gil, C. Fully Automated Determination of 3-MCPD and...