complete bone graft complete bone graft Rehydration of cancellous sponge with bone marrow aspirate introduces osteogenic materials contain living cellular components that are capable of forming bone. Components of Bone Marrow Aspirate bone marrow aspirate Mesenchymal Stem Cells (MSCs): Cells with potential to become bone cells Osteoblasts: Bone cells capable of forming bone tissue Growth Factors: Proteins that may induce bone cell formation Growth Factors cancellous SPONGE Osteoinductive materials are capable of inducing differentiation of MSCs into osteoblasts. Osteoconductive materials contain a surface that is favorable for osteoblast attachment. Demineralized Bone è Osteoinductive Potential Demineralized with Large Surface Area • Osteoconductive matrix for cellular attachment Bone soaked in acid to remove mineral content Removal of mineral content exposes trapped growth factors Growth factors act on MSC and induce formation into osteoblasts • orous structure of the cancellous sponge provides increased P surface area to facilitate cell proliferati