3D Cell Culture
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Your Power for Health 3D Cell Culture 3D in a fast and easy 2D workflow

3D Cell Culture in a 2D workflow Cell culture is an essential tool in drug discovery, tissue engineering, toxicology testing, stem cell research, as well as in basic research. In 2D, cultures can only mimic the conditions of physiological tissue to a limited extent, whereas cells in vivo are able to interact in a three-dimensional network. Therefore, results generated with 2D cultures may often be of limited relevance for predicting clinical effectiveness and toxicity, contributing to high attrition rates in the drug development process.1 Cell culturing in 3D enables the expression of...

CELLSTAR® Cell Culture Vessels with Cell-Repellent Surface In contrast to standard tissue culture surfaces which are optimised to enhance conditions for cell attachment, the CELLSTAR® cell-repellent surface has been developed to effectively prevent cell attachment. For the formation of e.g. spheroids or self-assembled spherical clusters used as 3D cell culture models, the cell-cell interaction must dominate over the interaction between the cells and the culture surface of containment. Cell culture vessels equipped with the Greiner Bio-One CELLSTAR® cell-repellent surface present an ideal...

Magnetic 3D Cell Culture The core technology of Greiner Bio-One’s Magnetic 3D Cell Culture is the magnetisation of cells with biocompatible NanoShuttle™-PL, nanoparticles (Ø ~ 50 nm) consisting of gold and iron oxide, mixed with Poly-L-Lysine. Cell magnetisation occurs when NanoShuttle™-PL is attached electrostatically to the cell membrane during an overnight static incubation, after which cells can be aggregated by magnetic force to form structurally and biologically representative in-vitro 3D models, either by levitation or bioprinting. By levitating cells from 6 well or 24 well...

Magnetic 3D Cell Culture - Applications NanoShuttleTM-PL: Biocompatible Nanoparticle (Au, Fe2O3 & Poly-L-Lys) A video showing the handling of the magnetic 3D cell culture products and the experimental workflow is available on our website: www.gbo.com/3dcellculture Magnetising Cells BioAssay (96 & 384 Well) with 3D ring structure Levitation (6 & 24 Well) Larger levitating spheroids Bioprinting (96, 384 Well & 1536 Well) Smaller spheroids for HTS Wound healing Compound screening Toxicity screening Toxicity screening Cardiovascular research Genomics and Protein Analysis Viability assays Figure...

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