Automated Solutions for HPV Genotyping
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PapilloCheck® Automated Processing Your Power for Health greiner bio-one Your Power for Health greiner bio-one PapilloCheck® HPV Genotyping CX™ NIMBUS® and CX™ STARlet Platforms - smart. efficient. accurate. CX™ NIMBUS® and CXTM STARlet enable a streamlined use of PapilloCheck®, the HPV genotyping kit from Greiner Bio-One. Both systems combine the advantages of automation with an outstanding methodology developed for the simultaneous screening and genotyping of human papilloma viruses: smart. 1. ready to use load & go reagents 2. simultaneous processing of different collection media 3. modular processing of extraction, PCR and hybridisation possible 4. magnetic bead separation technology 5. intelligent waste management efficient. 1. simultaneous HPV screening and genotyping 2. fully automated sample prep from primary tubes 3. cost & time savings due to increased lab staff productivity resulting from short hands-on time 4. LIMS compatible for automated result reporting 5. reduced tip consumption due to optimised work flow programming accurate. 1. robust DNA array technology 2. liquid level detection, aspiration and clot detection monitoring 3. built-in controls and quality control reporting 4. capable of performing daily/weekly instrument maintenance CXTM NIMBUS® performs DNA extraction and PCR setup for simultaneous analyses of up to 48 samples. The sealing of the PCR plate and the PCR are conducted with external devices of your laboratory. CXTM STARlet processes 96 samples per run, integrating DNA extraction, PCR set-up, PCR plate sealing and PCR. CX™ NIMBUS® plus and CX™ STARlet plus display an additional feature to enable hybridisation of the amplified PCR product onto the chip. Scanning and chip evaluation is detected using CheckScanner™ and the CheckReport™ Software. Fast and Reliable Genotyping of 24 Human Papillomavirus Types Persistent infection with a carcinogenic human papillomavirus (HPV) is found in virtually all cases of cervical cancer1-4. HPV testing is currently being investigated as the primary tool in cervical cancer screening. Cervical HPV types are classified into a high risk (hrHPV) and a low risk (lrHPV) group, with respect to the risk of progression from mild dysplasia to cancer5. Therefore, it is important to have a rapid and reliable diagnostic tool for the identification of HPV types in cervical samples. PapilloCheck® (CE-IVD) is a DNA-array based diagnostic tool for the simultaneous detection and genotyping of 24 different HPV types. Of these, 18 HPV types are classified as high risk and 6 HPV types as low risk, with the later types being the causative agent of benign warts6. PapilloCheck® - At a glance <5: High sensitivity and specificity Simultaneous genotyping and monitoring of 24 HPV types ^ Classification according to risk potential Integrated quality control system Validated according to international guidelines for HPV tests in cervical cancer screening PapilloCheck® - Detectable HPV types high risk [hrHPV] Due to the simultanous identification of the 24 detectable HPV types, the result from a PapilloCheck® analysis allows for the characterisation of multiple infections. It can help to identify type-specific persistent HPV infections and clarify the status of a patient in the course of HPV vaccination. Therefore, it has the potential to improve the classification of women according to their relative risk for developing cervical cancer and its high-grade precursor lesions. PapilloCheck® was validated according to international guidelines for HPV test requirements in cervical cancer screening. It meets the demands regarding clinical sensitivity and specificity as well as reproducibility6,7. As clinical studies have proven PapilloCheck® is a robust HPV genotyping assay with the potential for high throughput of specimens in a clinical setting8. PapilloCheck® samples can be processed manually as well as on various newly developed automation platforms which allow a high sample throughput with minimal manual handling. 1 Zur Hausen H. (2002), Nat Rev Cancer, 2(5):342-50. 2 Walnoomers J. et al., (1999), J. Pathol. 189:12-19. 3 Bosch X. & Iftner T (2005), NHSCSP Publication No. 22. 4 Burd EM. (2003), Clinical Microbiology Reviews, 16:1-17. 5 Munoz et al., (2003), N. Engl. J Med, 348:518-527. 6 Meijer, C.J. et al. (2009), Int J Cancer. 124(3):516-20. 7 Hesselink, A.T et al. (2010), J Clin Microbiol. 48(3):797-801 8 Jones J. et al. (2009), J Clin Virol. 45(2): 100-4.

HPV detection and genotyping with PapilloCheck® CXTM NIMBUS® can perform time-consuming pipetting steps that support routine use of Greiner Bio-One PapilloCheck® to enable in tandem use of other external devices (e.g. thermal cycler, sealer device). Hands-on time is reduced and productivity is increased, facilitating a cost-efficient solution for rapid workflow. CX™ NIMBUS® includes four pipetting channels, a CO-RE paddle system for a fast and safe transfer of plates, a heater shaker, magnetic separator and a cooling block for enzymes. CX™ NIMBUS® plus additionally integrates a...