This combined document includes a brochure for all of the following areas for Macherey Nagel: • MACHEREY NAGELAIugramXtra • MACHEREY NAGEL Derivatization reagents for GC • MACHEREY NAGEL GC Catalogue • MACHEREY NAGEL Optima 1301 MS • MACHEREY NAGEL GC Optima 5 HT • MACHEREY NAGEL GC Optima 17 MS • MACHEREY NAGEL GC Optima 35 MS • MACHEREY NAGEL GC Optima 1701 MS • MACHEREY NAGEL GC Optima 1-MS Accent/Optima 5-MS Accent/Optima XLB • MACHEREY NAGEL GC Optima FFAPplus • MACHEREY NAGEL GC Optima WAXblus Greyhound Chromatography and Allied Chemicals 6 Kelvin Park Birkenhead Merseyside. CH41 1LT Tel: 0151 649 4000 Fax: 0151 649 4001 Email: [email protected] Web: https /ftwww.greyhoundchrom com

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MACHEREY-NAGEL – About us Quality since 1911 Since 1911 MACHEREY-NAGEL stands for high quality, innovation and reliability in chemical and biomolecular analysis. Friendly expert advice for our highly valued customers as well as outstanding product quality have been the cornerstones of our corporate success for more than 100 years. MACHEREY-NAGEL Rapid Tests is a family-owned company run by the fourth generation. As one of today’s leading manufacturers of products for analytical chemistry and life science we ofer a broad range of products for Filtration, Rapid Tests, Water Analysis, Chromatography...

MACHEREY-NAGEL Chromatography - Complete solutions for your analysis MACHEREY-NAGEL has grown from a pioneer in chromatography to a full-range supplier of laboratory consumables. We supply laboratories all over the world with HPLC, GC and SPE columns, TLC plates and sheets, syringe filters or suitable vials and closures. Our philosophy includes personal and competent • Competent and individual service • More than 50 years of expertise in manufacturing of chromatographic adsorbents • Detailed product information and technical data can be found at www.mn-net.com • Online application database with...

Solid phase extraction

Basics Solid phase extraction (SPE) is a powerful method for sample preparation and is used by most chromatographers today. About 25 years ago MACHEREY-NAGEL designed and introduced CHROMABOND® SPE cartridges containing silica-based adsorbents. Since then we have developed the widest range of phases and products for SPE based on silica and polymeric materials. SPE has capabilities in a broad range of applications · Environmental analysis · Pharmaceutical and biochemical analysis In general, SPE is used for three important purposes in stateof-the-art analysis · Concentration of the analyte – up...

Moisturizing of the solid phase with the matrix Sample application Sample is pressed or drawn through the solid phase • Analyte molecules are enriched on the adsorbent • Interfering components and solvent molecules (matrix) are not retained • Remaining interfering components are washed from the adsorbent • The analyte is removed from the adsorbent by elution with a suitable solvent • Analyte molecules show no interaction with the adsorbent - Interfering components and solvent molecules (matrix) are retained • Analyte molecules are “washed" from the adsorbent • The solid phase is used to “filter"...

Basics Molecular interactions in SPE SPE adsorbents are most commonly categorized by the nature of their primary interaction mechanism with the analyte of interest. The three most common extraction mechanisms used in SPE are reversed phase (RP), normal phase (NP) and ion exchange. Typical extraction mechanisms · Reversed phase extraction of hydrophobic or polar organic analytes from aqueous matrix · Normal phase extraction of polar analytes from nonpolar organic solvents extraction of charged analytes from aqueous or nonpolar organic samples Types of retention mechanisms Nonpolar interactions...

Basics It should be noted, that in SPE the interactions described on page 12 are not found in pure form, but in combination. For example, modiied silicas, unless they have been subjected to endcapping (silanization of residual silanol groups with shortchain silanes), still possess free silanol groups, which can enter into secondary interactions. Sample pretreatment For direct extraction with adsorbents the sample matrix (sample environment) has to fulill three conditions: · The matrix has to be liquid, if possible with low viscosity · Solids should be removed from the liquid matrix · The matrix...

All CHROMABOND® columns, cartridges and 96-well plates are manufactured from polypropylene (PP) with lowest content of extractables (plasticizers, stabilizers, ...) offering blank value free results when using most common solvents. The high quality CHROMABOND® adsorbents are kept in place by chemically very inert polyethylene filter elements (PE, standard pore size 20 pm). CHROMABOND® LV CHROMABOND® MULTI 96 Sample reservoir polypropylene, 15 mL CHROMABOND® polypropylene columns • PP columns with PE filter elements • Different sizes from 1,3, 6 up to 150 mL • Adsorbent weights from 20 mg to 50...

CHROMABOND® hardware CHROMABOND® SPE columns from page 23 onwards CHROMABOND® Multi 96 page 14 and 69 CHROMABOND® Flash RS page 75 CHROMABOND® Flash BT page 76 CHROMABOND® Flash DL page 76 CHROMABOND® Flash FM page 77

CHROMABOND® summary of MN phases CHROMABOND® Phase Matrix Modification / Application Similar phases* Page Reversed phases HR-X PS/DVB Easy PS/DVB ENVI-Chrom P ■ Strata™-X ■ Oasis® HLB ■ Nexus polar, bifunctional Strata™-X ■ Oasis® HLB ■ Porapak™ RDX ■ Nexus, Bond Elut® PPL, Focus™ ■ Styre Screen® DVB Bakerbond™ H2O-philic DVB ■ Isolute® ENV+ Strata™ SDB-L ■ Bond Elut® ENV, Bond Elut® LMS ■ DSC-PS/DVB, ENV PS-DVB ■ Bakerbond™ H2O-phobic DVB ■ Isolute® 101 ■ LiChrolut® EN 30 removal of organic components octadecyl, endcapped Strata™ C18-E ■ Sep-Pak® tC18 ■ Bond Elut® C18 ■ DSC-18(Lt), ENVI-18,...