Product Overview: The 16S Metagenomics NGS Primer Pools, catalog number YST0079, are designed for research use to amplify hypervariable regions of the 16S rDNA gene in bacteria. This allows for accurate detection and identification of a wide range of bacteria at the genus or species level. The product includes two primer pools, A and B, and is available in various sizes as detailed on the company's website.

Applications: The primary application is 16S Metagenomics NGS, which is a method used in microbial community analysis.

Amplification Protocol: The protocol involves preparing a reaction mixture with the following components: 10 µL of 2X Ultra HiFi PCR Master Mix, 2 µL of either 10X Primer Pool A or B, a variable volume of DNA or stool sample (denoted as x), and water to make up the remaining volume to 20 µL. The total reaction volume is 20 µL.

PCR Cycling Parameters: The cycling parameters for PCR include an initial denaturation at 95℃ for 10 minutes, followed by 20 to 30 cycles of denaturation at 99℃ for 15 seconds and annealing/extension at 60℃ for 4 minutes.