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  3. YST0038-GTX-PCR-Master-Mix-with-ROX-Product-Insert_v1.pdf

YST0038-GTX-PCR-Master-Mix-with-ROX-Product-Insert_v1.pdf

YST0038-GTX-PCR-Master-Mix-with-ROX-Product-Insert_v1.pdf
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YST0038-GTX-PCR-Master-Mix-with-ROX-Product-Insert_v1.pdf

Product catalog summary
Product Overview: The document provides details about the 2X GTX PCR Master Mix with ROX, catalog number YST0038, designed for genotyping PCR using TaqMan end-read allelic discrimination assays. It is noted for its robustness and high performance, allowing the use of whole blood samples directly without DNA purification.
Applications: The product is suitable for genotyping assays from whole blood and other crude sample types without the need for DNA purification. It includes ROX for signal normalization.
Test Method with Crude Blood DNA:
  • Crude Blood DNA Preparation: Mix 20uL of Lysis Buffer A with 2uL of fresh blood in a 1mL tube. Vortex and incubate at room temperature for 3 minutes, then add 20uL of Lysis Buffer B to obtain the crude DNA sample for PCR.
  • PCR Reaction Setup (20uL):
    • 2X GTX PCR Master Mix: 10uL
    • 20X TaqMan Assay (Primer/Probe Mix): 1uL
    • DNA Sample: 2uL
    • Water: 7uL
    • Total Volume: 20uL
Thermal Cycler Protocol: The document mentions a thermal cycler protocol but does not provide specific details in the excerpt.
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Catalog excerpts

YST0038-GTX-PCR-Master-Mix-with-ROX-Product-Insert_v1.pdf-1

Product Insert Catalog # YST0038, 2X GTX PCR Master Mix with ROX. Check our website for the various product sizes available. A genotyping PCR Master Mix for TaqMan end-read allelic discrimination assays, very robust and high performance. Whole blood sample can be used directly without purification of DNA for genotyping. Application: Genotyping assays from whole blood and other crude sample types without DNA purification. It contains ROX for signal normalization. Test Method with Crude Blood DNA 1. Crude Blood DNA Preparation: Add 20uL Lysis Buffer A in a 1mL tube, and then add 2uL fresh blood. Vortex and incubate at room temperature for 3minutes. Add 20uL Lysis Buffer B. This is your crude DNA sample for PCR. 2. PCR for 20uL Reaction Volume: Component Volume, uL 2X GTX PCR Master Mix 20X TaqMan Assay (Primer/Probe Mix) Thermal Cycler Protocol: 425 Boulder Court, Suite 500, Pleasanton, CA 94566, USA Phone: 1+(510) 861-2542 Email: [email protected] www.sensecarebio.com FOR RESEARCH USE ONLY.

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