Product Insert Catalog # YST0053, 2X Fast SYBR Green Real-Time qPCR Master Mix with ROX. Check our website for the various product sizes available. Fast SYBR Green Real-Time qPCR Master Mix is a convenient SYBR Green dye–based PCR amplification and detection reagent in a single-tube format. This Master Mix contains SYBR Green I dye, DNA Polymerase, dNTPs, and optimized buffer. Product Features：    Fast SYBR Green I dye detects double-stranded DNA, so specific probes are not required Hot-Start DNA polymerase minimizes nonspecific product and primer-dimer formation Fast SYBR Green Real-Time PCR Master Mixt can be used for fast PCR instruments to deliver results within 30 minutes. Applications: DNA quantification by Real-Time PCR without using fluorescent probes and contains ROX for signal normalization. Test Method for 20uL Reaction Volume: Component 2X Fast SYBR Green PCR Master Mix with ROX Thermal Cycler Protocol with Dissociation Curve: 425 Boulder Court, Suite 500, Pleasanton, CA 94566, USA Phone: 1+(510) 861-2542 Email: [email protected]