Optical Oxygen Sensors
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The Real Cost of Dissolved Oxygen Measurement 80% of sensor costs come after the initial purchase Polarographic Sensors Traditional polarographic membrane sensors use a chemical reaction to generate a small electrical current that is correlated to oxygen concentration in the sample. Keeping the sensor in top condition requires skilled troubleshooting, expensive replacement parts, and considerable time waiting for the sensor to polarize. TROUBLESHOOT IN 3-8 HOURS POLARIZE TEST IN 100% AIR SERVICE CALIBRATE TROUBLESHOOT ELECTROLYTE MEMBRANE CAP ANODE/CATHODE

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CALIBRATING OPTICAL SENSORS One point, two point, and product calibrations can all be performed using existing polarographic procedures. Optical Sensors Optical sensors measure using an oxygen-sensitive fluorescent dye. The dye requires no equilibration time so it is instantly ready for use. A quality indicator displays the health of the sensor cap, and maintenance is as simple as removing and reinstalling the cap. TROUBLESHOOT IN ≤ 20 MINUTES WARM UP SERVICE CALIBRATE TROUBLESHOOT REPLACE SENSOR CAP

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Can You T rust Your Oxygen Measurement? Are polarographic and optical measurements comparable? Yes, when run in parallel, the data gathered from optical and polarographic sensors is often identical. However, below is one example of when the readings differ. Can you tell which sensor is reading accurately? OPTICAL SENSOR POLAROGRAPHIC SENSOR The graph above shows data from a side-by-side comparison of optical (monitoring) and polarographic (controlling) values over a long fermentation run. The divergence at the end of the run is a result of CO2 fouling of the polarographic control sensor,...

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WHAT IS CO2 FOULING? Over long fermentation runs, sensors are exposed to CO2 gas used for pH control or resulting from cellular respiration. The gas travels through the membrane of a polarographic sensor and changes the pH of the electrolyte. This change results in a lower nA reading. CO2 Respired by Cells CO2 Enters Sensor, Forms H2CO3 and Neutralizes the Electrolyte Pressure Spikes Improved mechanical and measurement stability with a glass H2S window instead of a membrane Signal is not influenced by electrostatics and H2S mechanical stress No impact on measurement or sensor life

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Intelligent Sensors Eliminate more than costly transmitters The integrated micro transmitter eliminates signal noise and enables predictive estimates of sensor life, calibration and troubleshooting. These can be conducted in a controlled lab or at line, and documentation is simplified with automatic report generation. WIRELESS CALIBRATION & DIAGNOSTICS Automatic Calibration & GMP Documentation Sensor Status Warnings Errors

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Optional Wireless Adapter Reduced Calibration Costs Eliminate Costly Transmitters Galvanic Isolator For enhanced signal quality Integrated Micro Transmitter Improved Signal Reliability Simplify wiring to the control system

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The Visi Family of Sensors The right tool for the job The optical VisiFerm sensors are available in a variety of configurations to meet the needs of your challenging application. The core measurement principal is consistent across all models with various electrical connections, sensor caps, and firmware versions to ensure superior performance and reliability. SIGNAL PROCESSING & TRANSMISSION VisiFerm DO Seamless integration with existing analog bioreactors and transmitters Output: ECS, 4-20 mA, and Modbus outputs VisiFerm DO Arc Improve signal with direct connection to the control system...

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The fastest response time and compatibility with most fermentation and culture media Strengthened luminophore matrix for better temperature stability with fast response time. The perfect cap for most biopharmaceutical applications Only compatible with the VisiFerm mA sensor Chemically resistant with hygienic design to simplify cleaning and minimize bubble accumulation H4 Cap Strengthened luminophore matrix for better temperature stability with chemically resistant and hygienic design L0 Trace Cap Optimized for trace measurement from 1 to 2,000 ppb and stable against active chlorine and...

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Unprecedented Connectivity Eliminate the middle man; talk directly to the sensor New installations are abandoning dedicated transmitters in favor of computer screens. The Visi family of sensors transitions seamlessly with analog options for existing installations and a variety of digital protocols for future needs. CALIBRATION & DIAGNOSTICS Wirelessly communicate with the sensor via Bluetooth. Setup, troubleshoot, and calibrate multiple sensors from a single handheld device or PC.

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REPLACE TRADITIONAL OXYGEN SENSORS Validated Cables for Most Bioreactors’ Bioreactor Manufacturer: ► Sartorius ► Bioengineering Bioreactor Manufacturer: ► Applikon ► DASGIP (Eppendorf) Enjoy the benefits of optical oxygen measurement with your existing equipment. VisiFerm DO can simulate the output of a traditional polarographic sensor (ECS Mode, Electrochemical Signal) for integration into any system with minimal effort. Bioreactor Manufacturer: ► Applikon Bioreactor Manufacturer: ► New Brunswick (Eppendorf) All Cables have integrated power supplies with selectable country specific plugs...

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© 2017 Hamilton Company. All rights reserved. All trademarks are owned and/or registered by Hamilton Company in the U.S. and/or other countries. L30011/02 Hamilton Americas & Pacific Rim Hamilton Company Inc. 4970 Energy Way Reno, Nevada 89502 USA Tel: +1-775-858-3000 Fax: +1-775-856-7259 sales@hamiltoncompany.com Hamilton Europe, Asia & Africa Hamilton Bonaduz AG Via Crusch 8 CH-7402 Bonaduz, Switzerland Tel: +41-58-610-10-10 Fax: +41-58-610-00-10 contact.pa.ch@hamilton.ch To find a representative in your area, please visit hamiltoncompany.com/contacts.

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