BioLevitator? - 16 Pages

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Catalog excerpts

rotativeLoiB rotativeLoiB The Future of 3D Biology, Unveiled

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3D Cell Culturing Developed for the Bench-top GEM™ Control The LeviTube™ and gnirutluC lleC D3 gnirutluC lleC D3 rof depoleveD rof depoleveD pot-hcneB eht pot-hcneB eht designed to gently suspend the GEM culture to ensure cells have access to media. Magnets The BioLevitator is a bench-top incubator and bioreactor hybrid capable of handling four BioLevitator are used to pellet independent and high density 3D cell cultures. It the cells for media exchange and harvesting. eliminates peripheral instruments such as incubators and centrifuges, and minimizes manual handling. The BioLevitator...

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User Interface Precise Environmental Control The touch-screen interface provides users with intuitive and easy to program cell culture The compact incubator chamber is designed to monitor and maintain CO2 and temperature workflows. In addition, the ergonomic click-wheel allows quick navigation. during cell expansion. Culture Reports, including metrics such as pH, can be viewed real-time or exported for record keeping and compliance purposes. Data Logging Transfer data via USB memory stick from the BioLevitator™ to the PC. » next See how it works with the GEM™

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Dissolution Solution Dissolves alginate to a liquid releasing the bound cells ™MEG ehT ™MEG ehT ti sekaM ti sekaM elbissoP elbissoP More Than Just a Microcarrier Specialty protein coatings facilitate the attachment and expansion of cell types that are difficult or impossible to grow using traditional methods. Cells excrete nutrients into the alginate core where they remain biologically available, allowing the use of animal free media. Why GEM? The GEM is designed to maintain the unique in vivo phenotype of your preferred cell line while providing a vehicle for culture transport through the...

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Magnetic Particles Enables pelleting of cells for media exchange Protein Coat Gelatin Laminin Basement Membrane Fibronection Collagen I Collagen IV Custom Coatings Available Cell Excretion Cells release nutrients into the electro negative core Nutrients are available after media exchange 75 – 150 µm Enables pipeting of GEM™ cultures

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Grow Happier & Healthier Cells & reippaH worG & reippaH worG slleC reihtlaeH slleC reihtlaeH Including Primary and Stem Cells The goal of the BioLevitator™ is to enable scientists to perform experiments with more relevant cell models. Instead of growing transformed cell lines, scientists will now be able to grow normal cells that more closely mimic in vivo characteristics. Cells cultured in a 3D environment grow at higher densities and form structures that more closely resemble cells taken from living subjects. With the BioLevitator researchers have the tools necessary to design high...

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Primary & Stem Cells Human Proximal Tubule Human Adipose Stem Cell Established Cell Lines Visit for more information Cancer Cells Prostate Cancer Cell (PC3) Bladder Cancer Cells (T24)

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Overall Savings of 62% Using the BioLevitator ™ ,robaL ni %26 evaS ,robaL ni %26 evaS rotativeLoiB eht gnisU rotativeLoiB eht gnisU Improve Yearly Cost Savings $10,000 $9,000 $8,000 What could you accomplish with 45 weeks free from cell culture? By using the ‘Cells as Reagent’ model (as shown on page 10) you do not have to continuously culture cells —saving you crucial time and money. Start Saving after the First Two Months Time (Months) Note: Cost Savings Based on Weekly Consumption for 12 months. Based on usage of 40 million cells per week and the cells as a reagent model. The cell type...

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Scale-up The cost effective solution for every stage of research. The BioLevitator™ Provides a Cost Effective Solution for Every Stage of Cell Production Growing more cells in the BioLevitator increases the savings. Laboratories that transition from growing 40 million cells to 320 million cells per week can see a 3-fold increase in savings per year relative to traditional 2D methods. Cost Savings (BioLevitator vs. 2D Methods) Number of Cells Cultured Per Week (Millions) Note: Based on a weekly consumption for 12 Months. The cell type used was CHO with 1 mL of GEM™ at a 50% slurry per...

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Accelerate Cell Expansion in 3D lleC etareleccA lleC etareleccA D3 ni noisnapxE D3 ni noisnapxE Generate more cells and use them at your pace with “Cells as a Reagent” Cell Expansion One 50 mL LeviTube™ equals the cell growth of up to ten T75 flasks. (0.5 mL GEM™ at 50% solution) Note: Typical for CHO, HEK293, HeLa cell types. Cell Density The GEMs spherical design provides more surface area for culturing cells. 7.6-fold more MDCK cell growth on the same surface area. 200 180 160 MDCK Cells (Per Unit Area)

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Cell Storage Eliminate just-in-time cell production with cryopreservation. Cell Viability GEM™s inhibit formation of ice crystals and minimize exposure to harsh chemicals like DMSO. Cells as a Reagent Step 1. Manufacture cells and store them in aliquots. Step 2. Thaw an aliquot. Step 3. Ready to use! Passage & Scale up Harvest Spin, Count & Plate Automation Consistent Biology from small scale to high-throughput screening. Small Scale 10 Million Cells / week Medium Throughput 400 Million Cells / week High Throughput 2 Billion Cells / week 3D CellHost™ (Houses up to 4 BioLevitator units)

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Simplifying Cell Based Assays lleC gniyfilpmiS lleC gniyfilpmiS syassA desaB syassA desaB Skip harvesting and go straight to the assay with a relevant cell model 3D Cell Culturing Using the BioLevitator and GEM™ Technology. Extra step involved when using the conventional method. Confluent Flask Cellular Assay Cell Harvesting & Recovery Note: The GEM can be easily dissolved if your experiments require. Cellular Assay Relevance More in vivo Phenotype Human Renal Proximal Tubule cells (hRPTCs) show a more in vivo-like phenotype when attached to the GEM as opposed to 2D cell culture where the...

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