CHECK THE KIT CONTENTS BEFORE USE The test device is sealed and should be stored away Positive (+): The presence of both C line and T line, regardless of T line being strong or faint. from light at a room temperature (4-30℃). Do not Bovine Pregnancy Rapid Test (PAG) freeze. The test device should be used before the expiration date marked on the package label. Warnings, Precautions and Safety Information 1. The test device is used for bovine only. Disposable dropper Package insert 2. The results may be influenced by humidity and Negative (-): Only clear C line appears. Invalid: No colored line appears in C region, regardless of T line’s appearance. through rectal palpation or ultrasound. With advancements in biological sciences, it has been discovered that Pregnancy-Associated specific proteins that appear in the peripheral blood of ruminants after conception, secreted by binucleate cells of the embryo, and play a crucial role during pregnancy. Our rapid bovine early pregnancy test card offers an accurate method for detecting PAGs in whole blood (EDTA), plasma (EDTA), or serum as early as 26 days post-breeding and throughout the entire pregnancy. This diagnostic tool is invaluable for veterinarians, dairy farms, and beef production facilities, aiding in reducing 3. Make sure that the foil pouch containing the test is not damaged before open. Perform the test immediately when the pouch package is opened. Check the product contents and make sure the test operation is under the room temperature (15-30℃) before testing. 4. Do not reuse the test components. 5. Do not use after the expiry date. 6. Do not mix product components in different lot TEST PROCEDURE Although the Bovine Pregnancy Rapid Test is very accurate in 7. As all samples are potentially infectious. Operators should wear protective gloves while handling samples incidence of false results may be occurred. Other clinically or and wash hands thoroughly afterwards. laboratory tests might be required if questionable results are 8. Decontaminate and dispose of all samples, used kits obtained. As other diagnostic tests, a definitive clinical and potentially contaminated materials safely in diagnosis should not be based on the result of a single test, but accordance with national and local regulations. S Traditionally, pregnancy in cattle has been diagnosed should be diagnosed by the veterinarian after all clinical and laboratory findings have been evaluated. breeding cycles, increasing breeding success rates, and controlling breeding costs. Specimen Collection, Handling, and Transport The Bovine pregnancy Rapid test is a qualitative test. It detects presence (PAGs) ruminants after pregnancy using a double-antibody sandwich system on a nitrocellulose membrane. During the test, the PAGs in the blood of cattle binds with a gold label and then binds with the antibody on the test line, showing a purple band. If the sample does not contain PAGs, no color reaction occurs. 1.Whole blood, serum or plasma should be used with this test. contains sufficient for tests in vitro diagnotic medical device temperature limitation Whole blood: Collect the whole blood. If whole blood samples are not immediately tested, they should be refrigerated at 2～8°C and used within 24 hours. Serum: Collect the whole blood into the collection tube (NOT containing anticoagulants such as heparin, EDTA and sodium citrate), and then centrifuge whole blood to get serum. Plasma: Collect the whole blood into the collection Tear open the aluminum foil bag and take out the test card, placing it on a flat surface. Use a dropper to add one drop of specimen and three drops of buffer to the sample well. (If the whole blood has not begun to react after about two minutes, add 1～2 additional drops of buffer.) and sodium citrate) and then centrifuge whole blood to get plasma. INTERPRETATION OF TEST RESULT 2.Samples should be stored at 2～8°C. Please freeze · Test devices · Disposable droppers the samples at ‐20°C or below for longer storage and · Package insert avoid repeated freezing and thawing. 3.Samples containing precipitate may yield inconsistent Materials Required but not Provided test results. They must be clarified prior to assaying. erroneous results. 4.Hemolyzed or contaminated samples may lead to Hangzhou Antigenne Technology Co., Ltd Address：Block A, Building 3, No. 8, Xiyuan 9th Road, Xihu District, Hangzhou, Zhejiang, 310030 P.R. China tube (containing anticoagulants such as heparin, EDTA Reagent and Materials Provided