7. As all samples are potentially infectious. Operators should wear protective gloves while handling samples Abnormal level (+): If the color intensity of the T1 line is equal to or darker than the color intensity of the reference T2 line, CPL levels are abnormal. TEST PROCEDURE and wash hands thoroughly afterwards. Canine Pancreatic Lipase Rapid Test（CPL） 8. Decontaminate and dispose of all samples, used kits and accordance with national and local regulations. Unseal the extraction tube containing the buffer. Place the extraction tube in the workstation. Normal level (-): If the color intensity of the T1 line is lighter than the color intensity of the reference T2 line, CPL levels are normal. Specimen Collection, Handling, and Transport C T2 T1 Invalid: No colored line appears in C region, regardless of T line’s appearance. Canine Pancreatic Lipase Rapid Test is a lateral flow immunoassay intended for the determination of 1.Serum or plasma should be used with this test. Canine Pancreas-Specific Lipase Levels (CPL) in Serum: Collect the whole blood into the collection tube canine serum or plasma. (NOT containing anticoagulants such as heparin, EDTA and sodium citrate), and then centrifuge whole blood to get serum. Reagent and Materials Provided · Test devices · Quantitative droppers · Buffer tubes · Package insert Materials Required but not Provided Timer Plasma: Collect the whole blood into the collection tube (containing anticoagulants such as heparin, EDTA and sodium citrate) and then centrifuge whole blood to Squeeze the upper air bag to absorb the sample (serum or plasma) . Please make sure some sample getting into the lower air bag and there is no bubbles in the lower tube. Then press the upper air bag to transfer the sample (about 75 μL) left in the lower tube into the buffer. Although the Canine Pancreatic Lipase Rapid Test is very 2.Samples should be stored at 2~8°C. Please freeze accurate in detecting Canine Pancreas-Specific Lipase Levels, the samples at ‐20°C or below for longer storage and a low incidence of false results may be occurred. Other clinical- avoid repeated freezing and thawing. ly or laboratory tests might be required if questionable results 3.Samples containing precipitate may yield inconsistent are obtained. As other diagnostic tests, a definitive clinical test results. They must be clarified prior to assaying. diagnosis should not be based on the result of a single test, but should be diagnosed by the veterinarian after all clinical and 4.Hemolyzed or contaminated samples may lead to erroneous results. Cover the tube, Jiggle the extraction tube until the specimen and the buffer are mixed completely. laboratory findings have been evaluated. Storage and Stability The test device is sealed and should be stored away batch code contains sufficient for tests in vitro diagnotic medical device temperature limitation from light at a room temperature (4-30℃). Do not freeze. The test device should be used before the expiration CHECK THE KIT CONTENTS BEFORE USE Warnings, Precautions and Safety Information date marked on the package label. Take the test device out of the aluminum foil bag, and place it on a clean, flat table. Add three drops (about 90 μL) of the mixed sample vertically into the specimen well (S) of the test device. 1. The test device is used for canine only. 2. The results may be influenced by humidity and damaged before open. Perform the test immediately 4. Do not reuse the test components. 6. Do not mix product components in different lot numbers. INTERPRETATION OF TEST RESULT Test device Quantitative Buffer tube Package insert Workstation when the pouch package is opened. 5. Do not use after the expiry date. Address：Block A, Building 3, No. 8, Xiyuan 9th Road, Xihu District, Hangzhou, Zhejiang, 310030 P.R. China temperature. 3. Make sure that the foil pouch containing the test is not Hangzhou Antigenne Technology Co., Ltd Check the product contents and make sure the test operation is under the room temperature (15–30℃) before testing.