7. As all samples are potentially infectious. Operators should wear protective gloves while handling samples Canine Relaxin Rapid Test （C.RLN） TEST PROCEDURE and wash hands thoroughly afterwards. Although the Canine Relaxin Rapid Test is very accurate in 8. Decontaminate and dispose of all samples, used kits detecting Canine Relaxin hormone, a low incidence of false results may be occurred. Other clinically or laboratory tests accordance with national and local regulations. might be required if questionable results are obtained. As other diagnostic tests, a definitive clinical diagnosis should not be based on the result of a single test, but should be diagnosed by the veterinarian after all clinical and laboratory Specimen Collection, Handling, and Transport Intended Use Canine Relaxin Rapid Test is a lateral flow immunoassay 1.Serum or plasma should be used with this test. intended for the qualitative detection of relaxin hormone Serum: Collect the whole blood into the collection tube in canine serum or plasma sample. Canine Relaxin (NOT containing anticoagulants such as heparin, EDTA Rapid Test is intended to determine pregnancy in a and sodium citrate), and then centrifuge whole blood to bitch as well as to distinguish between pseudopregnan- cy and a real gestation.Test no earlier than 28 days post Plasma: Collect the whole blood into the collection tube (containing anticoagulants such as heparin, EDTA and sodium citrate) and then centrifuge whole blood to get plasma. Reagent and Materials Provided 2.Samples should be stored at 2~8°C. Please freeze the samples at ‐20°C or below for longer storage and avoid · Test devices · Buffer tubes · Disposable droppers repeated freezing and thawing. · Package insert 3.Samples containing precipitate may yield inconsis tent test results. They must be clarified prior to Materials Required but not Provided Timer findings have been evaluated. on a clean, flat table. Use a dropper to draw some serum/plas- Hangzhou, Zhejiang, 310030 P.R. China INTERPRETATION OF TEST RESULT Read the result at 5-10 minutes. The result is invalid after 15 minutes. Positive (+): The presence of both C line and T line, regardless of T line being strong or faint. Negative (-): Only clear C line appears. 2. The results may be influenced by humidity and temperature. damaged before open. Perform the test immediately Test device Disposable dropper Buffer tube Package insert when the pouch package is opened. Invalid: No colored line appears in C region, regardless of T line’s appearance. 4. Do not reuse the test components. 5. Do not use after the expiry date. Check the product contents and make sure the test 6. Do not mix product components in different lot operation is under the room temperature (15–30℃) before Address：Block A, Building 3, No. 8, Xiyuan 9th Road, Xihu District, 3. Make sure that the foil pouch containing the test is not from light at a room temperature (4-30℃). Do not 1. The test device is used for canine only. temperature limitation Hangzhou Antigenne Technology Co., Ltd The test device is sealed and should be stored away Warnings, Precautions and Safety Information in vitro diagnotic medical device (about 60 μL) of buffer into the specimen well (S) of the test CHECK THE KIT CONTENTS BEFORE USE contains sufficient for tests well (S) of the test device, and then immediately add two drops erroneous results. ma specimens, add one drop (about 30 μL) into the specimen 4.Hemolyzed or contaminated samples may lead to date marked on the package label. Take the test device out of the aluminum foil bag, and place it The test device should be used be