PatchServer: Main advantages • Automatic establishment of all patch clamp recording configurations (whole-cell and excised patch), utilizing standard glass pipettes and a computer controlled, direct feedback-guided approach Less training Easier, faster, and more reliable than manual patch clamp Simple handling High data quality User interrupt possibilities Automated process can be stopped in any phase Visual identification and evaluation of cells Easy switch to manual patching, if desired Modular add-on system Complete PatchServer automated patch clamp setups can be offered Existing setups can be equipped with the PatchServer for automation One channel system can be upgraded to four channels Multiple, individual recordings One channel version increases ease of use Four channel version additionally increases throughput Standard glass pipettes instead of expensive chips Efficient cell usage -- User can visually identify cells morphologically or with fluorescence before they are patched. This insures that recordings are made only from valid cells, saving time and resources. Part of the unused cells are returned to the CellReservoir, before the recording configuration is established. Less than 500 cells are wasted per recorded cell. Pipette-Based Automatic Patch Clamp System • Makes manual patch clamp easier and more efficient • Cells can be visually evaluated before the experiment • Automated cell supply and Giga-Ohm sealing Developed in cooperation with the Natural and Medical Sciences Institute (NMI), Reutlingen, Germany © October 2017 Multi Channel Systems MCS GmbH Product information is subject to change without notice. Products that are referred to in this document may be either trademarks and/or registered trademarks of the respective owners. The publisher and the author make no claim to these trademarks. Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Phone +49-7121-9 09 25 25 Fax +49-7121-9 09 25 11 [email protected] www.multichannelsystems.com • Automatically establishes whole-cell recording configuration • Works with standard glass pipettes • Highest data quality at low running costs •

PatchServer: Manual patch clamp goes automatic The PatchServer is an automatic patch clamp system that can establish whole-cell recording or excised- The PatchServer executes all steps fully automatically and requires patch configurations using tools and techniques from the manual patch approach. minimal user intervention. It utilizes standard glass electrodes and employs step-by-step procedures that would be applied by human experimenters as well. The PatchServer combines advantages from the automatic and manual patch clamp approach, respectively. Recording pipettes automatically locate Automation...

PatchServer: Manual patch clamp goes automatic The PatchServer is an automatic patch clamp system that can establish whole-cell recording or excised- The PatchServer executes all steps fully automatically and requires patch configurations using tools and techniques from the manual patch approach. minimal user intervention. It utilizes standard glass electrodes and employs step-by-step procedures that would be applied by human experimenters as well. The PatchServer combines advantages from the automatic and manual patch clamp approach, respectively. Recording pipettes automatically locate Automation...

PatchServer: Main advantages • Automatic establishment of all patch clamp recording configurations (whole-cell and excised patch), utilizing standard glass pipettes and a computer controlled, direct feedback-guided approach Less training Easier, faster, and more reliable than manual patch clamp Simple handling High data quality User interrupt possibilities Automated process can be stopped in any phase Visual identification and evaluation of cells Easy switch to manual patching, if desired Modular add-on system Complete PatchServer automated patch clamp setups can be offered Existing setups can...