Group: HORIBA
Catalog excerpts
ELEMENTAL ANALYSIS FLUORESCENCE GRATINGS & OEM SPECTROMETERS OPTICAL COMPONENTS FORENSICS PARTICLE CHARACTERIZATION Low Cost Phosphorescence Lifetime LRET System RAMAN SPECTROSCOPIC ELLIPSOMETRY SPR IMAGING An exceptionally fast, sensitive and accurate luminescence spectrometer for LRET assays and general phosphorescence Advantages of Lanthanide-Based Probes Chelated lanthanide probes offer some distinct advantages over conventional organic fluorophores. Lanthanides exhibit luminescence lifetimes ranging from hundreds of microseconds to milliseconds, which are orders of magnitudes higher than typical fluorescence lifetimes. By utilizing a pulsed excitation source combined with time-resolved detection, and selecting only the long-lived emission from the lanthanide ion, one can easily eliminate the background signal due to native or impurity fluorescence and scattered light, thus greatly enhancing the sensitivity and accuracy of an assay. This has led to development of numerous assays based on Luminescence Resonance Energy Transfer (LRET), which utilizes chelated lanthanide donors rather than organic fluorophores. Features and Benefits • Very fast single-shot decay acquisition • Ideal for LRET assays and phosphorescence • Ultra-high sensitivity and acquisition speed • Very high precision • High stability and reproducibility • Instant lifetime determination • Time-based lifetime scan • Time-based intensity scan • Lifetime temperature ramping • Intensity temperature ramping • Reduced bleaching • Low maintenance Key Applications • Rare earth phosphorescence • LRET-based kinase activity assays • Conformational changes in ligand-binding glutamate receptor • Lanthanide doped semi-conductors • Semiconducting nanocrystals • Sigma factor binding to RNA polymerase • K+ channel voltage sensor movement in cell membranes • Detection of salicylic acid in blood by sensitized Tb luminescence • Assays for determining antibiotics (norfloxacin, garenoxacin, grepafloxacin) in urine and serum • DNA-lanthanide assays for multi-drug resistance of TB strains DNA-lanthanide assays are used in a number of diagnostic tests, for example DNA-Tb3+ in testing for antibiotic resistance of tuberculosis strains. Terbium luminescence is enhanced upon binding to DNA, which res
Open the catalog to page 1Unique Acquisition Protocol EasyLife™ L Offers Special Features Due to its unique ‘single-shot’ detection technique, the EasyLife™ L is lightening fast. It can acquire a complete decay from a single flash of the 1 ps pulsed xenon lamp. Decays can be instantly analyzed in real time enabling the user to follow reaction kinetics by plotting the lanthanide lifetime rather than the intensity as a function of time. This is the basis for a new, unique feature that only the EasyLife™ L can offer: the Time-based Lifetime Scan. This makes the experiment immune to artifacts that usually affect...
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