FluoroMax Series Sensitive, flexible, easy-to-use fluorometers Powerful technology with bench-top convenience

“With unmatched sensitivity and unparalleled flexibility, the FluoroMax Series can meet all of your lab’s experimental needs.” FluoroMax Series The FluoroMax series represents HORIBA's industry-leading spectrofluorometer performance in a convenient, affordable, easy to use bench-top model. The FluoroMax family, with its unique all reflective optics and photon counting, was the first to bring the sensitivity of a modular fluorometer to a tabletop unit. Today the FluoroMax series consists of the FluoroMax-4, the latest generation of the original, high performance tabletop fluorometer, and the new...

Maximizing signal, Minimizing noise the sensitivity of one instrument to another with the multiple specifications and differing definitions that pervade. Sensitivity of a spectrofluorometer is generally reported as a minimum measureable concentration of a fluorophore (commonly Fluorescein), or the signal- to-noise ratio (S/N) of the Raman scattering peak of water. Most companies opt for the second, as it is a more readily verified number, especially in the field. Unfortunately, however, this specification can be confusing since two calculation methods prevail in the industry: First Standard Deviation...

Dynamic Range Measure more than 6 orders of magnitude of signal levels in the same spectrum Fortunately not all samples are weak. Some samples are quite strong. But some are both, which creates a challenge to measure the strong and weak peaks in the same scan. With an intra-spectral range of over 6 orders of magnitude, even wildly varying multicomponent spectra are no problem to measure in one scan. Not only does this save time, but it is essential for kinetics studies when one cannot afford to repeat a measurement at different integration times to keep all peaks within range. Spectrum of a mixture...

Measuring temporal variations in fluorescence spectra can yield information beyond that available from single time point measurements. For example, monitoring protein stability and folding in controlled solution conditions is crucial for developing targets for structure-based medicines. The FluoroMax series lets you measure dynamics on a scale of Kinetics of Lysozyme dissolved in several concentrations of the chaotrope; Guanidiniurn Hydrochloride, monitored by measuring native Tryptophan fluorescence simultaneously for 1 hour in a multi-sample changer. Data corrected for photobleaching using...

Adding optional polarizers to a FluoroMax allows measuring anisotropy, and hence, the changes in sample rotation time. This indirect measure of the local viscosity gives information on sample aggregation, structural changes, molecular binding, and other mechanisms. Absorbance/Transmittance Accessory Add absorbance to your fluorescence measurement. Correct your fluorescence spectra for innerfilter effects in concentrated samples without dilution. Reabsorption of fluorescence photons can occur even at moderate concentrations, and lead to increasing distortion of spectra with increasing concentration...

Microvolumes Measure ultra-low sample volumes with easy sample recovery HORIBA’s Microsense, the most advanced microliter accessory on the market, is designed to get cuvettequality steady state or lifetime data from a 1-5 µL of sample. Avoiding dilution and nearly total recovery minimizes the need for sample, while preserving measurement sensitivity. Based on all quartz optics, Microsense is compatible with UV to NIR measurements. Using the Microsense, 5 µL of AlexaFluor 488 labeled IgG is enough to get steadystate or TCSPC data. Well Plate Accessory Maximize your throughput Search for “hits”...

Full Spectral Microscopy Couple a microscope to a FluoroMax and: • Measure a complete spectrum of a sample as small as 1 µm. • Get spectra from as little as a few molecules of sample. • Perform localized FRET measurements. Add an automated stage and camera, and: • Create complete spatial/spectral maps. • Perform repetitive QC characterization of structured samples like photovoltaics. Enhanced Measurements Data from a real and forged US 5 dollar bill. Fluorescence imaging shows no obvious differences. However it is easy to spectrally distinguish the real from a fake bill. All instruments in the...

Peltier-based Heated/Cooled Cuvette Holders Rapid temperature control in single or multiple cuvette models with built-in magnetic stirring. Precise temperature control for precise data on your protein folding, micellization, solubility, conformation, phase, and rotational transitions. Rapidly vary sample temperature over a range of -40 to 110°C. FluoroMax software also simplifies automating temperature dependence measurements, including complex ramps and profiles. Temperature Bath An alternative to Peltier-based units. Better precision (0.01°C), range (-25 to 200°C) and long term stability, but...

Cryogenic temperatures enable measurements of fine structure, enhanced phosphorescence, and rare conformations/states often not possible at room temperature. This low cost accessory readily permits measurements of samples at 77°K. Economic LN Temperature Measurement Dewar Accessory Temperature can add thermal broadening to fluorescence spectra and increase phosphorescence quenching. Spectra of Naphthalene dissolved in Methanol measured at room temperature (298°K) and in a liquid nitrogen dewar accessory (77°K). The low temperature spectrum reveals rich vibrational structure and longer wavelength...

Other Accessories The FluoroMax series includes the most comprehensive line of accessories that enable researchers to extend the utility of their instrument to as many experiments as possible. The following is a partial list of accessories available, in addition to those previously discussed. • Auto-titrator (injector) dual syringe, dual valve. • Hi-Tech SFA-20 Stopped Flow, rapid kinetics accessory. • Solid Sample Holder designed for viewing front-face fluorescence of thin films, powders, pellets, paper, fibers, or microscopic slides. Variable alignment angle. • 2-position sample holder with...