Comprehensive Tumor Profiling HTG EdgeSeq DLBCL Cell of Origin Assay

HTG provides multiplexed gene expression assays and automation platforms that leverage the power and sensitivity of next-generation sequencing (NGS). Designed to empower precision medicine, HTG's extraction-free chemistry provides more data from small samples, such as a single FFPE tissue section including core needle biopsies. Recent advances in diffuse large B-cell lymphoma research have shown that determining the subtype is important for patient enrichment in clinical trials. Unlike traditional methods like immunohisto-chemistry (IHC), the HTG EdgeSeq DLBCL Cell of Origin Assay enables researchers...

The HTG EdgeSeq DLBCL Cell of Origin Assay provides classification of Diffuse Large B-Cell Lymphoma (DLBCL) tumors into the activated B-cell like (ABC), germinal center B-cell like (GCB) or "unclassified" subtype using a single 5-micron FFPE tissue section including core needle biopsies. Sample The sensitivity and dynamic range of next-generation sequencing paired with the specificity of HTG EdgeSeq chemistry enables gene expression measurement with minimal sample input. Profile challenging sample types, including FFPE tissue, to deliver more data from less material. Applications include: ■ Interrogate...

Profiling and Subtyping in a Single Assay Diffuse large B-cell lymphoma is the most common non-Hodgkin lymphoma (NHL), accounting for 30-40% of all cases. The 2016 updates to the World Health Organization (WHO) lymphoid neoplasm guidelines require classification of DLBCL tumors into the ABC or GCB subtype. Classification and profiling from a single FFPE tissue section inclusive of a core needle biopsy. Case in Point A pharmaceutical company was working from a hypothesis that their drug was more effective in one DLBCL subtype, but could not justify the testing of their clinical specimens due to...

Tumor Surface Area per Slide HTG Gene Expression Profiling Competitor Hans IHC 5 pm thickness Recommended Minimum Recommended Minimum HTG Recommended sample input for competitive DLCBL COO assays versus the HTG EdgeSeq DLBCL Cell of Origin Assay Expression of PDCD1andCD274 (PD-1 and PDL-1) was consistent across the same titrations, indicating that relatively low expression genes are detected using the HTG EdgeSeq DLBCL Cell of Origin Assay in small FFPE tissue inputs Sample Input COO Subtype Sample Input COO Subtype ■ ABC Subtype Consistent DLBCL cell of origin subtype classification was obtained...

HTG EdgeSeq DLBCL COO Assay Illumina 916-003-008 HTG EdgeSeq DLBCL Cell of Origin Assay (4x8) HTG EdgeSeq DLBCL Cell of Origin Assay (4x24) HTG EdgeSeq DLBCL Cell of Origin Assay (1x96) HTG EdgeSeq system HTG EdgeSeq processor HTG Edge system PC and accessories Gene List Lymphoma Markers Drug Targets For Research Use Only. Not for use in diagnostic procedures.

VERI/O Lab Services Our in-house VERI/O laboratory services support biomarker research and companion diagnostic development using HTG’s extraction-free technology and portfolio of profiling assays. Planned services are expected to include direct sequencing of genetic variants. CLIA-Certified Partners For prospective research, HTG has partnered with several CLIA-certified laboratories that provide profiling services using HTG systems and assays. Contact your HTG representative for more information. Partner with HTG Commitment Comprehensive, NGS-based molecular profiling is a critical tool that...

HTG Molecular Diagnostics, Inc. 3430 E. Global Loop, Tucson, AZ 85706 Customer Support: +1 877 507 3259 Corporate Office: +1 877 289 2615 +1 520 547 2834 Fax: +1 520 547 2837 Service and support: [email protected] Orders: [email protected] More information: [email protected] HTG Edge and HTG EdgeSeq processors are CE-marked and CSA-certified. For Research Use Only. Not for use in diagnostic procedures. HTG EdgeSeq, HTG Edge and qNPA are trademarks of HTG Molecular Diagnostics, Inc. Any other trademarks or trade names used herein are the intellectual property of their respective...