HTG EdgeSeq System Workflow Brochure

Product catalog summary

Overview: The HTG EdgeSeq system is a molecular profiling tool designed for biopharmaceutical companies, academic research centers, and translational medicine laboratories. It offers an automated workflow solution using HTG's extraction-free chemistry, compatible with next-generation sequencing (NGS) to produce gene expression profiles in approximately 36 hours.

Workflow and Chemistry: The system's workflow includes several key steps: sample preparation, library preparation, next-generation sequencing, and data parsing. The entire process from sample to answer takes about 36 hours, with specific time allocations for each step, such as 4 hours for quantitation and normalization, and 20 hours for target protection.

Sample Preparation: The HTG EdgeSeq system uses a lysis-only chemistry, allowing small sample inputs without nucleic acid extraction. This simplifies the preparation process, reducing time and potential errors. The system includes all necessary components like lysis buffers and proteinase K.

Library Preparation: The process involves adding nuclease protection probes to the lysed sample, forming a heteroduplex with the target RNA. This is followed by S1 nuclease treatment to remove non-hybridized probes and RNA, preparing the sample for sequencing.

Next-Generation Sequencing: The system is compatible with common NGS instruments and uses HTG Edge host software to generate expression data. The setup is quick, taking less than 30 minutes for an Illumina MiSeq sequencer run.

Data Parsing: The HTG Edge host software provides a user-friendly interface for data parsing and display. The system demonstrates high reproducibility across NGS platforms, with a Pearson correlation greater than 0.98.

Additional Information: The HTG EdgeSeq processor is CE-marked and CSA-certified, intended for research use only. For more information, contact HTG Molecular Diagnostics.

Catalog excerpts

HTG EdgeSeq System Sample to Answer in 36 Hours

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HTG EdgeSeq System Workflow and Chemistry Overview Sample to answer in 36 hours The HTG EdgeSeq system is a powerful molecular profiling tool for biopharmaceutical companies, academic research centers and translational medicine laboratories. The HTG EdgeSeq system provides an automated workflow solution based on HTG’s extraction-free chemistry. The HTG EdgeSeq system is used with next-generation sequencing (NGS) instrumentation to produce gene expression profiles in about 36 hours. Sample Preparation Library Preparation Target Protection ~10 minutes hands-on time ~30 minutes hands-on time Plasma/...

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For more information contact HTG Molecular Diagnostics at 1-877-289-2615, [email protected] or contact your local HTG Molecular Diagnostics representative. Visit www.htgmolecular.com HTG Molecular Diagnostics, Inc. 3430 E. Global Loop, Tucson, AZ 85706, USA Call 1-877-289-2615 Fax 1-520-547-2837 [email protected] Service and Support 1-877-507-3259 Orders [email protected] HTG EdgeSeq processor is CE-marked and CSA-certified. For Research Use Only. Not for use in diagnostic procedures. In the U.S. and other applicable jurisdictions, HTG EdgeSeq is a trademark of HTG Molecular Diagnostics,...

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HTG EdgeSeq System Workflow Brochure

HTG EdgeSeq System Workflow Brochure

Catalog excerpts

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