RealTime ready Custom RT-qPCR Assays and Panels for any Human, Mouse, or Rat Gene

RealTime ready qPCR Assays and Panels RealTime Ready Configurator Search by Focus Panel Search by Pathway Ordering Information For life science research only. Not for use in diagnostic procedures. License Disclaimer: A license to perform the patented 5’ Nuclease Process for research is obtained by the purchase of (i) both Licensed Probe and Authorized 5’ Nuclease Core Kit, (ii) a Licensed 5’ Nuclease Kit, or (iii) license rights from Applied Biosystems. This product contains Licensed Probe. Its purchase price includes a limited, non-transferable immunity from suit under U.S. Patent No. and 5,804,375...

RealTime ready Complex Gene Expression Analysis of Human, Mouse, and Rat Genes, Made Simple aximize efficiency M Select assays for your targets of choice from gene lists of functionally related gene families or biological pathways. Order them as single assays or configure your own custom panel on a LightCycler® 480 Multiwell Plate. nsure optimal results E Rely on function tested qPCR assays based on proven Universal ProbeLibrary technology, with detailed bioinformatic background information and novel error-detection features. et the complete picture G Discover the full functionality of your targets...

RealTime ready qPCR Assays and Panels Get a Head Start on Your Gene Expression Analysis RealTime ready RT-qPCR Assays for any human, mouse, or rate gene of your choice are based on highly specific, LNA-substituted short hydrolysis probes from the Universal ProbeLibrary (UPL). Rigorously tested on the LightCycler® 480 Instrument with species-specific universal biological samples, each assay is experimentally qualified to perform according to strict criteria derived from the MIQE* guidelines. inear dynamic range of at least 3 logs L CR efficiency values of 90% to 110% P ² value of standard curve...

Assay Design Characteristics RealTime ready Assays are designed using the proprietary ProbeFinder Software, which is based on optimized and verified algorithms. For a particular gene, one representative transcript is chosen for assay design. The selection of this transcript is based on annotation and cross-references within two of the main public gene/transcript annotation resources, Ensembl and Entrez Gene. This procedure ensures comprehensive coverage of most known splice variants of a certain gene. Most RealTime ready Assays are intron-spanning, enabling reliable quantification unbiased by genomic...

RealTime ready Custom Panels for the LightCycler® 480 System Custom panels on LightCycler® 480 Multiwell Plates 96 or 384 are composed of RealTime ready Assays of your choice. A multitude of different layouts (see Figures 1 and 2) is available for configuration. Assays are pre-plated and dried down for a reaction volume of 20 pl on 96-well plates and 10 pl on 384-well plates; simply add sample cDNA and LightCycler® 480 Probes Master for fast and reliable results. On each panel, up to three wells are reserved for reference gene assays of your choice, matching the organism you have selected for...

The RealTime ready Configurator online design tool features an intuitive graphical interface and a large variety of layouts (with or without controls) that can be filled in with assays of your choice. Your configuration progress is automatically stored in your passwordprotected account at every step; your panel content will be available for download after your purchase is completed. Browse the Configurator at www.configurator.realtimeready.roche.com. Search Functions Assay selection and configuration of custom panels is easy with the comprehensive search functions of the RealTime ready Configurator. ■...

RealTime ready Focus Panels The RealTime ready Configurator features Focus Panels with pre-selected assays for genes from a variety of pathways, functional groups, and gene superfamilies. The table below shows a selection of Focus Panels, grouped into different research topics and biological pathways. Angiogenesis Apoptosis Caspase Activation Execution of Apoptosis Induction of Apoptosis Negative Regulation of Apoptosis Positive Regulation of Apoptosis Apoptosis - Selection 384 Apoptosis - Selection 96 Proteolytic Processes During Apoptotic Cell Death DNA Cleavage During Apoptotic Cell Death Cell...

Use the Configurator to browse the contents of each of the gene lists below, and use the “Search by Focus Panel” function to create your own custom panel with function tested assays. You can either load a complete gene list to your custom panel or create your own selection by picking the targets from one or more Focus Panels. Immune Response Oncology Signal Transduction Apoptosis FLT3 Signaling GPCR Signaling Hemostasis Inhibition of Angiogenesis Immune System Innate Immunity Integrins Interleukin Family Interleukin Receptors JNK Signaling NF-kB Signaling NF-kB Target Genes Signaling in...

The “Search by Pathway” function allows you to select your targets from a variety of interactive maps of cellular or signaling pathways. These maps immediately reveal possible interactions of your selected targets with other genes. Simply click on the genes of interest to add them to your assay search.

Roche Applied Science offers a large selection of reagents and systems for life science research. For a complete overview of related products and manuals, visit and bookmark our homepage, www.roche-applied-science.com, and our Special Interest Sites including: RealTime ready Custom Assays and Panels: http://www.realtimeready.roche.com The Universal ProbeLibrary: http://www.universalprobelibrary.com The LightCycler® System family for real-time PCR: http://www.lightcycler.com Amplification - Innovative tools for PCR: http://www.roche-applied-science.com/pcr Trademarks: LIGHTCYCLER, REALTIME READY,...

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