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- ABDOMINAL ORGAN PERFUSION SYSTEMS
ABDOMINAL ORGAN PERFUSION SYSTEMS
ABDOMINAL ORGAN PERFUSION SYSTEMS
This document provides an overview of perfusion systems used in animal physiology research, particularly focusing on isolated organ systems. It emphasizes the modular design of these systems, which allows for customization to meet specific research needs. The systems are engineered to maintain homeostasis in isolated organs by controlling temperature, pH, and aeration, utilizing a perfusion pump.
The document details various perfusion systems tailored for different organ systems, including gastrointestinal, urogenital, vascular, bronchial, cardiac, skeletal muscle, and nervous systems. These systems are designed for specific research applications such as studying metabolic processes, drug effects, and transplantation.
Key components of an isolated perfused organ system include a base system, perfusate pump, thermocirculator, buffer reservoirs, species-specific cannulae, transducers, and data acquisition methods. These components work in unison to ensure the health and functionality of the isolated organ during experiments.
Perfusate is moved using an analog peristaltic pump, with a windkessel to dampen pulsations. The Servo Controller (SCP) maintains constant flow and pressure, monitored by a pressure transducer and displayed digitally. Data acquisition software records physiological parameters such as pO2, pH, and pCO2.
Moist chambers provide a controlled environment for organ perfusion, featuring superior temperature control and compatibility with various accessories. They are used for perfusion of abdominal organs and are suitable for long-term studies, including drug testing and transplantation research.
These chambers are designed for sterile conditions, with features like interchangeable parts, built-in bubble traps, and autoclavable materials. They are ideal for long-term studies involving regenerative tissue engineering and organ transplantation.
Hugo Sachs Elektronik and Harvard Apparatus offer comprehensive solutions for isolated organ research, supported by expert technical assistance. The document provides detailed specifications and applications for various perfusion systems, ensuring researchers can select the optimal setup for their studies.
This multipurpose system is designed for perfusing isolated organs either ex vivo or in situ. It supports constant flow or pressure perfusion and is suitable for organs like the liver, kidney, and mesenteric bed. The system is modular, allowing for easy adaptation to different applications.
The document describes the setup for in situ perfusion, including the use of an operating table and heat exchanger to maintain consistent perfusate temperature. Additional equipment like a thermocirculator and peristaltic pump is required.
These tables are available in heated and homeothermic versions, suitable for small animal surgeries. They include features like paw holders and options for thorax retractors. The tables are designed to maintain optimal surgical access and temperature control.
These chambers are used for perfusing isolated pig liver and kidney, maintaining physiological temperature conditions. They allow for constant flow or pressure perfusion and are used in transplantation studies.
Designed for studying perfused tubular organs, this system accommodates intraluminal and extraluminal perfusions. It requires two peristaltic pumps and includes adjustable cannulae for different organ lengths.
This system is used for isolating cells from organs like the heart and liver through enzymatic disintegration. It features dual perfusion circuits for blood cell flush and enzyme solution perfusion, with options for constant flow or pressure perfusion.
The document provides detailed specifications for each system, including dimensions, power supply requirements, and additional equipment needed for operation.
The PSCI-M and RSCI-R systems are designed for cell isolation from mouse and rat organs, respectively. They include a dual heat exchanger and a platform for a peristaltic pump, which are adjustable and secured to a stainless steel rod. The systems can be adapted for ex vivo or in situ perfusion by adding specific components like a jacketed moist chamber or an operating table.
The system is used for isolating primary cells from organs by perfusion, initially with a perfusate for blanching and then with a protease solution for tissue disintegration. It is suitable for cardiomyocyte isolation and can be configured for different organs.
The document details various peristaltic pumps, including the Harvard Peristaltic Pump and REGLO Analog & Digital Pumps. These pumps offer features like high accuracy, ease of use, and connectivity to external devices. They support a wide range of flow rates and tubing sizes, with options for analog and digital interfaces.
The document also describes centrifugal and pulsatile blood pumps designed for laboratory use, emphasizing low hemolysis and suitability for various fluid types. These pumps are robust and designed for long-term operation, with features like magnetic coupling and minimal noise.
The BVP-ZX pump drive offers adjustable speeds from 3 to 3000 rpm and supports various analog interfaces for speed control. It is compatible with the BP-80 centrifugal pump head, which provides a maximum flow rate of 16 L/min at 50 mmHg.
The SCP is designed for precise perfusion control, maintaining constant pressure or flow by adjusting the peristaltic pump speed. It requires a measuring system for perfusion pressure or flow and an analog pump capable of external control.
Models like TC120, TX150, and TXF200 offer accurate temperature control with varying temperature ranges and stability. The LT ecocool series provides energy-efficient options with significant cost savings.
Jacketed reservoirs are used with pumps to deliver warmed perfusate. They are available in standard, sealing, or with drain options, and require separate tubing sets for interfacing with thermocirculators.
Fiber oxygenators, available in D150 and D200 sizes, use MediSulfone membranes for effective oxygenation. They are suitable for blood or perfusate containing protein and come with various mounting options.
Designed for atraumatic cannulation, these cannulae are available in multiple sizes for different species and organs, featuring basket-like tips to prevent occlusion.
The Pump 11 Elite series offers precise flow control with options for single or dual syringes. It is suitable for drug addition and various studies, featuring a user-friendly touchscreen interface.
The document lists software and hardware options for data acquisition, including PowerLab systems with LabChart software for comprehensive data analysis.
PowerLab® systems, combined with LabChart™ software, provide comprehensive data acquisition and analysis capabilities for research applications. The systems are available in various configurations, offering 4, 8, or 16 channels, and are suitable for a wide range of physiological measurements.
BDAS is designed for measuring slow-moving signals such as temperature and pH. It supports up to 16 channels and allows user-defined parameters and analysis algorithms.
LabChart™ offers advanced features for data recording and analysis, supporting up to 16 channels. It is suitable for various species and research applications, providing modules for ECG, blood pressure, and more.
PLUGSYS is a modular system for physiological data acquisition, offering a wide range of modules for different measurements. The system supports easy interconnection and integration with various amplifiers and recording devices.
Pressure transducers like the APT300 and P75 are used to monitor arterial and venous pressures in isolated organ setups. They ensure constant pressure to prevent edema and are compatible with PLUGSYS modules.
This system continuously measures pH, pO2, and pCO2 in perfusion solutions, crucial for drug studies and physiological experiments.
The TTFM-2 is used for real-time flow measurement in blood or perfusates, featuring ultrasonic technology and compatibility with various flow probes.
The document provides specifications for various flow probes and thermocouple thermometers. Flow probes are categorized by type with inner diameters ranging from 1 mm to 8 mm and nominal flow ranges from 20 ml/min to 2 L/min.
The document outlines the use of the PLUGSYS TTFM-2 Module for measuring blood flow in various species and the PLUGSYS Thermocouple Amplifier Module (TCAM) for temperature monitoring.
Various accessories are listed, including mini ball joint holders, lab stands, surgical kits, and magnetic stirrers.
For isolated organ systems, a checklist is provided to help configure an ideal setup, including options for organs, species, perfusion modes, and measurement systems.
Information on other perfused organ systems, such as isolated perfused heart and lung systems, is available, with references to brochures and technical services for detailed information.
The document concludes with a list of references to scientific studies related to the equipment and applications discussed, providing additional context and validation for the products.
Catalog excerpts
Abdominal Organ PERFUSION SYSTEMS Liver • Kidney • Mesenteric Bed Intraluminal Vessel Perfusion www.hugo-sachs.de www.harvardapparatus.com
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PERFUSION & TISSUE BATH SYSTEMS OVERVIEW Solutions for All Aspects of Animal Physiology Research Organs Smooth Muscle GASTROINTESTINAL TRACT • • • • Esophagus Stomach Liver/Pancreas Intestine Systems for Organs/Applications • Moist Chamber—perfusion of liver, pancreas • UP-100—perfusion of liver ex vivo or in situ • PBTO—intraluminal intestine perfusion • CP—perfusion of GI organs and tissues using S peristaltic pump • IPR—perfusion of ileum peristaltic reflexes UROGENITAL TRACT • Kidney • Placenta VASCULAR MUSCULATURE • Hind Quarter • Mesenteric Bed • Coronary Vasculature • Moist Chamber—perfusion...
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Abdominal ORGAN PERFUSION S Y S T E M S Hugo Sachs Elektronik (HSE), part of the Harvard Bioscience family of companies, provides top-notch, fully integrated physiology research systems, including perfusion and tissue bath systems for many organ and tissue types. This catalog contains all the information you need to choose an optimal isolated abdominal organ system for your research. These comprehensive and complete systems are modular in design, providing the flexibility to tailor a system to fit your specific needs. Whether you need a complete isolated organ system or an addition to a system...
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CONFIGURING AN ISOLATED PERFUSED ORGAN SYSTEM Typical setup of complete Autoclavable Moist Chamber System rgans within the body are designed to perform different, specialized functions that work in unison to maintain homeostasis in a living organism. Despite their differences, similar conditions are required to study organs in isolation. These requirements include controlled temperature, a nutrient or buffer at physiological pH, aeration for the buffer, a perfusion pump to move the buffer and a system with a chamber to hold the isolated organ. Some organs may require additional equipment related...
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Perfusion Pressure Perfusion Flow Portal Vein Roller Pump Transducer Amplifier Module Waste Perfusate is pumped from the reservoir into the portal vein or hepatic artery (not shown) using an analog peristaltic (roller) pump. A windkessel is installed in order to dampen the roller pump’s pulsations. Perfusion pressure and perfusion flow can be adjusted and are measured. Constant flow and constant pressure perfusion can be performed by the Servo Controller (SCP). A pressure transducer (P) together with the TAM-D constantly monitors the perfusion pressure. The perfusion flow is calculated from the...
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KIDNEY PERFUSION VIA RENAL ARTERY Data Acquisition Dissolved Oxygen Dissolved Carbon Dioxide Renal artery Renal vein Ureter Windkessel Perfusion Pressure Transducer Amplifier Module Perfusion Flow Roller Pump Perfusate is pumped from the reservoir into the renal artery using an analog peristaltic (roller) pump. A windkessel is installed in order to dampen the roller pump’s pulsations. Perfusion pressure and perfusion flow are kept constant by the Servo Controller (SCP). A pressure transducer (P) together with the TAM-D constantly monitors the perfusion pressure. The perfusion flow is calculated...
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Moist Chamber Features & Benefits • Superior temperature control of perfusate and organ • Precise positioning of cannulae and measurement probes • Compact and easy to use • Compatible with a variety of accessories, making it suitable for a wide range of applications • Provides a complete perfusion system when combined with the UP-100 or a perfusion control system Applications • Rodent isolated organ perfusion-liver, kidney, pancreas, mesenteric bed • Investigation of the tone of small blood vessels under the effect of vasoactive substances • Biochemistry-studying metabolic processes • Drug studies-testing...
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Base Systems Autoclavable Moist Chamber Features & Benefits • Interchangeable connecting parts for the 16 openings around the chamber allows you to customize the chamber to suit your needs • Built-in bubble trap • Optional perfusate reservoir and oxygenator • Multiple cannulae sizes available to suit a variety of applications • Each perfusion line is equipped with an independent heat exchanger Applications • Long term drug studies on ex vivo liver, kidney, and other abdominal organs • Regenerative tissue engineering studies involving decellularization and recellularization with stem cells lasting...
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Moist Chamber with Edema Balance Features & Benefits • Dual perfusion system-vascular and intraluminal intestinal • Built-in balance for edema evaluation and organ weight measurement Applications • Rodent isolated intestine perfusion • Microvascular permeability studies • Simultaneous study of vascular, luminal and lymphatic flows • Arterial, venous and intraluminal pressures and bowel weight • Septic multi-organ failure studies in gastrointestinal area This system is comprised of a moist chamber with a built-in organ weighing system. The jacketed chamber maintains a warm and moist environment...
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Universal Perfusion System (UP-100) Features & Benefits • Multipurpose system for perfusing isolated organs ex vivo or in situ • Ideal for perfusing isolated organs such as: liver, rabbit ear, heart, kidney, rat hind limb, mesenteric bed • Allows for constant flow or constant pressure perfusion Applications • Ex vivo perfusion of liver, kidney, mesenteric bed (requires the addition of a moist chamber) • Test of vasodilative drugs • Studying metabolic processes • Neural vascular tone • Organ preservation for transplant • In situ perfusion of liver, kidney, mesenteric bed, hind limb, hindquarter...
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Operating Tables for In Situ Perfusion Studies Features & Benefits • Heated and homeothermic versions available • Includes paw holders • Capable of receiving thorax retractors and mounting ball joint holders Applications • For in situ perfusion studies, when paired with the UP-100 Universal Perfusion System • For general surgical work on small animals There are three heated options available for this operating table: • Basic Fixed Temperature: includes an adapter box and power supply. The temperature is fixed at 38°C. • Bdvanced Fixed Temperature: includes heating controller. Allows the user...
Open the catalog to page 11All Hugo Sachs Elektronik catalogs and technical brochures
BEHAVIORAL RESEARCH100 Pages
SURGICAL SOLUTIONS FOR RESEARCH260 Pages
ISOLATED LUNG PERFUSION SYSTEMS62 Pages
- Patient monitor
- Blood pressure patient monitor
- Compact patient monitor
- Medical gas flow meter
- Hospital flow meter
- Tabletop patient monitor
- Air flowmeter
- Induction chamber
- Non-invasive patient monitor
- Veterinary patient monitor
- Laboratory stereotactic frame
- Rodent laboratory stereotactic frame
- Rail-mounted flow meter
- Bench-top laboratory stereotactic frame
- Anesthetic gas scavenging system
- Anesthesia induction chamber
- Digital laboratory stereotactic frame
- Mice laboratory stereotactic frame
- Veterinary anesthesia induction chamber
- Rat laboratory stereotactic frame