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CARDIOMYOCYTE ISOLATION SYSTEMS
CARDIOMYOCYTE ISOLATION SYSTEMS
The document provides detailed information on cardiomyocyte isolation systems designed for small rodent hearts, specifically from mice to small rabbits. It covers two main systems: the EasyCell Simple Cell Extraction System and the PSCI Perfusion System for Cell Isolation.
- Design Features: A simple constant flow perfusion system for isolating cardiomyocytes, adaptable for other small rodent organs. It is easy to set up, operate, and maintain.
- Temperature Control: Includes a water-jacketed heat exchanger and heart chamber to maintain physiological temperature.
- Pulsation Dampening: An air vessel/compliance chamber dampens perfusion pressure pulsation, serving as a bubble trap to prevent air emboli.
- Operation: The system uses a single perfusion pathway compatible with ethanol for disinfection. It involves perfusing the heart with a warmed, aerated buffer followed by a protease solution for tissue disintegration.
- Included Items: The system includes various components such as a base stand, bubble trap, heating coil, protease reservoir, heart chamber, thermocirculator, and peristaltic pump.
- Design Features: A dual perfusion system for blood cell flushing and enzymatic digestion, compatible with ethanol disinfection. It has a low priming volume to conserve collagenase and minimize temperature loss.
- Perfusion Hub: Ensures thermal stability and laminar flow with bubble traps to prevent air emboli.
- Operation: The system releases individual cells through enzyme solution perfusion. It includes a jacketed heart chamber and a custom changeover stopcock for switching between clearing and collagenase buffers.
- Included Items: The core system includes a base unit, thermocirculator, glass reservoir, tube set, peristaltic pump, magnetic stirrer, and aortic cannula.
- Both systems are used for cardiomyocyte isolation and can be adapted for other organs such as liver for hepatocyte isolation.
- Details on product numbers and descriptions for ordering the EasyCell and PSCI systems are provided, with options for different voltage requirements.
Catalog excerpts
HUGO SACHS ELEKTRONIK a division of Harvard Bioscience, Inc. Cardiomyocyte Isolation Systems For Mouse to Small Rabbit Heart www.hugo-sachs.de • www.harvardapparatus.com
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EASYCELL SIMPLE CELL EXTRACTION SYSTEM DESIGN FEATURES • Simple constant flow perfusion system designed for cardiomyocyte isolation from small rodent hearts • Can be adapted for other small rodent organs in situ or ex vivo • Easy to set up, operate and maintain • Wetted parts can be disinfected with 70% ethanol or autoclaved for sterilization PULSATION DAMPENING • Air vessel/compliance chamber dampens perfusion pressure pulsation generated by the peristaltic pump • Serves as a primary bubble trap to prevent air emboli from causing regional ischemia, increasing potential cell yield TEMPERATURE...
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EasyCell System for Cardiomyocyte Isolation The EasyCell system has been specifically developed to isolate primary cells from hearts ranging from mouse to guinea pig. The system is supplied complete with everything you need for basic cardiomyocyte isolation. Perfusion pressure monitoring can easily be added to aid in choosing an appropriate physiological perfusion pump flow setting. The system can also be configured for either in situ or ex vivo organ perfusion with the addition of an operating table or moist chamber and appropriate cannulae. Applications • Cardiomyocyte isolation from small...
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PSCI PERFUSION SYSTEM FOR CELL ISOLATION PSCI PERFUSION SYSTEM FOR CELL ISOLATION • Dual perfusion system for blood cell flushing and enzymatic digestion • Compatible with disinfection using ethanol • Low priming volume conserves collagenase and minimizes temperature loss through tubing (<3 ml for mouse system, <5 ml for rat system) • Integrated platform for peristaltic pump minimizes system footprint and conserves bench space PSCI Perfusion System for Cell Isolation DESIGN FEATURES • Multi-purpose system can be fitted for use with other organs (e.g. in situ or ex vivo hepatocyte isolation from...
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PSCI PERFUSION SYSTEM FOR CARDIOMYOCYTE ISOLATION PSCI Perfusion System for Cell Isolation PSCI Perfusion System for Cardiomyocyte Isolation Operation The PSCI works such that individual cells are released from the tissue stucture through perfusion with enzyme solution and are then flushed out. For cardiomyocyte isolation, a jacketed heart chamber is mounted on a slide which is clamped to the vertical column. A slow gas flow into this chamber ensures a positive pressure inside the heart chamber at all times, thus preventing ingress of bacteria from the surroundings during operation. The cardiomyocytes...
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PSCI PERFUSION SYSTEM FOR CARDIOMYOCYTE ISOLATION Included items are representative of a typical PSCI Core System. Individual components can be customized to your needs. web www.hugo-sachs.de web www.harvardapparatus.com email [email protected]
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Cardiomyocyte Isolation Systems For Mouse to Small Rabbit Heart www.hugo-sachs.de · www.harvardapparatus.com
Open the catalog to page 7All Hugo Sachs Elektronik catalogs and technical brochures
BEHAVIORAL RESEARCH100 Pages
SURGICAL SOLUTIONS FOR RESEARCH260 Pages
ISOLATED LUNG PERFUSION SYSTEMS62 Pages
- Patient monitor
- Blood pressure patient monitor
- Compact patient monitor
- Medical gas flow meter
- Hospital flow meter
- Tabletop patient monitor
- Air flowmeter
- Induction chamber
- Non-invasive patient monitor
- Veterinary patient monitor
- Laboratory stereotactic frame
- Rodent laboratory stereotactic frame
- Rail-mounted flow meter
- Bench-top laboratory stereotactic frame
- Anesthetic gas scavenging system
- Anesthesia induction chamber
- Digital laboratory stereotactic frame
- Mice laboratory stereotactic frame
- Veterinary anesthesia induction chamber
- Rat laboratory stereotactic frame