CARDIOMYOCYTE ISOLATION SYSTEMS
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CARDIOMYOCYTE ISOLATION SYSTEMS - 1

HUGO SACHS ELEKTRONIK a division of Harvard Bioscience, Inc. Cardiomyocyte Isolation Systems For Mouse to Small Rabbit Heart www.hugo-sachs.de • www.harvardapparatus.com

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CARDIOMYOCYTE ISOLATION SYSTEMS - 2

EASYCELL SIMPLE CELL EXTRACTION SYSTEM DESIGN FEATURES • Simple constant flow perfusion system designed for cardiomyocyte isolation from small rodent hearts • Can be adapted for other small rodent organs in situ or ex vivo • Easy to set up, operate and maintain • Wetted parts can be disinfected with 70% ethanol or autoclaved for sterilization PULSATION DAMPENING • Air vessel/compliance chamber dampens perfusion pressure pulsation generated by the peristaltic pump • Serves as a primary bubble trap to prevent air emboli from causing regional ischemia, increasing potential cell yield...

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CARDIOMYOCYTE ISOLATION SYSTEMS - 3

EasyCell System for Cardiomyocyte Isolation The EasyCell system has been specifically developed to isolate primary cells from hearts ranging from mouse to guinea pig. The system is supplied complete with everything you need for basic cardiomyocyte isolation. Perfusion pressure monitoring can easily be added to aid in choosing an appropriate physiological perfusion pump flow setting. The system can also be configured for either in situ or ex vivo organ perfusion with the addition of an operating table or moist chamber and appropriate cannulae. Applications • Cardiomyocyte isolation from...

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PSCI PERFUSION SYSTEM FOR CELL ISOLATION PSCI PERFUSION SYSTEM FOR CELL ISOLATION • Dual perfusion system for blood cell flushing and enzymatic digestion • Compatible with disinfection using ethanol • Low priming volume conserves collagenase and minimizes temperature loss through tubing (<3 ml for mouse system, <5 ml for rat system) • Integrated platform for peristaltic pump minimizes system footprint and conserves bench space PSCI Perfusion System for Cell Isolation DESIGN FEATURES • Multi-purpose system can be fitted for use with other organs (e.g. in situ or ex vivo hepatocyte isolation...

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PSCI PERFUSION SYSTEM FOR CARDIOMYOCYTE ISOLATION PSCI Perfusion System for Cell Isolation PSCI Perfusion System for Cardiomyocyte Isolation Operation The PSCI works such that individual cells are released from the tissue stucture through perfusion with enzyme solution and are then flushed out. For cardiomyocyte isolation, a jacketed heart chamber is mounted on a slide which is clamped to the vertical column. A slow gas flow into this chamber ensures a positive pressure inside the heart chamber at all times, thus preventing ingress of bacteria from the surroundings during operation. The...

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CARDIOMYOCYTE ISOLATION SYSTEMS - 6

PSCI PERFUSION SYSTEM FOR CARDIOMYOCYTE ISOLATION Included items are representative of a typical PSCI Core System. Individual components can be customized to your needs. web www.hugo-sachs.de web www.harvardapparatus.com email support@hbiosci.com

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CARDIOMYOCYTE ISOLATION SYSTEMS - 7

Cardiomyocyte Isolation Systems For Mouse to Small Rabbit Heart www.hugo-sachs.de · www.harvardapparatus.com

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