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AC Broth Base

AC Broth Base
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AC Broth Base(For the test of Enterococcus) Uninoculated Code Directions Suspend 50.5 g of the dehydrated medium together with 0.25 g of Sodium azide in 1,000 mL of distilled water, mix well and heat to dissolve the medium. Distribute 10 mL of medium into the middle size of test tubes. Sterilize by autoclaving at 121 °C for 15 minutes. In case of sample volume is bigger than 10 mL, two or three fold concentration broth is prepared according to the volume of sample. For preliminary confirmation test of Enterococcus in water or foodstuffs, incubate at 37 °C for 48 hours. For confirmation test, incubate at 45 °C for 48 hours.(for assessment of MPN). Remarks For an identification of Enterococcus obtained from clinical samples, fish a suspected colony on the isolation culture, and inoculate to incubate at 45 °C for 48 hours. On the broth all gram negative and positive bacteria except Enterococcus are inhibited. Consequently, Enterococcus (+) is confirmed when any growth (turbidity) is visually observed. HyServe GmbH & Co.KG, Hechenrainerstr. 24, 82449 Uffing, Germany; www.hyserve.com; info@hvserve.com produced by Nissui Pharmaceuticls co., ltd

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AC Broth Base 1Pages

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AC Broth Base
1Pages