CW Agar Base with Kanamycin
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Catalog excerpts

CW Agar Base with Kanamycin - 1

Directions Suspend 60.0 g of the dehydrated medium in 900 mL of distilled water, mix well and heat to dissolve the medium. The heating should be done for less than 90 minutes, Maintain the medium at about 50 ° C, add 100 mL of the egg yolk saline suspension. Mix well and distribute about 20 mL into Petri dishes. When a C. perfringens Differentiation Strip is used, place the stick into the center of medium before it solidifies. To prepare a 100 mL of egg yolk solution, mix about 20 g of egg yolk with 80 mL sterilized physiological saline solution. Determinations After drying the surface of the plate, smear the unheated material, and incubate for 15 - 20 hours under anaerobic condition. Clostridium welchii (perfringens) forms yellowish white, round, convex colonies with a glistering surface and surrounded by a pearly ray zone (opacity of the medium). On the medium added with defibrinated blood instead of egg yolk, Clostridium welchii (perfringens) forms colonies of the same type with a glistering surface and a hemolytic ring. When the colonies are exposed to the air in an incubator, they will turn green. Formula HyServe GmbH & Co.KG, Hechenrainerstr. 24, 82449 Uffing, Germany; www.hvserve.com; info@hvserve.com produced by Nissui Pharmaceuticls co., ltd

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