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HyServe GmbH & Co. KG
Catalog excerpts
Directions Suspend 60.0 g of the dehydrated medium in 900 mL of distilled water, mix well and heat to dissolve the medium. Sterilize by autoclaving at 121 °C for 15 minutes. Maintain the sterilized medium at about 50 °C, add 100 mL of the egg yolk saline suspension or defibrinated blood. Mix well and distribute about 20 ml into Petri dishes. To prepare a 100 mL of egg yolk solution, mix about 20 g of egg yolk with 80 mL sterilized physiological saline solution. Determinations After drying the surface of the plate, smear the heated material, and incubate for 15 -20 hours under anaerobic condition. Clostridium welchii (perfringens) forms yellowish white, round, convex colonies with a glistering surface and surrounded by a pearly ray zone (opacity of the medium). On the medium added with defibrinated blood instead of egg yolk, Clostridium welchii (perfringens) forms colonies of the same type with a glistering surface and a hemolytic ring. When the colonies are exposed to the air in an incubator, they will turn green. HyServe GmbH & Co.KG, Hechenrainerstr. 24, 82449 Uffing, Germany; www.hyserve.com; info@hvserve.com produced by Nissui Pharmaceuticls co., ltd
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