EF Agar Base
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EF Agar Base - 1

EF Agar Base(Appended reagent: TTC, For Isolation of Enterococcus) Code Directions Suspend 54.5 g of the dehydrated medium together with 0.25 g of Sodium azide in 1,000 mL of distilled water, mix well and heat to dissolve the medium. Keep at around 60 °C, and then add 10 mL of 1.5% TTC solution. Mix well and distribute the medium into Petri dishes. 1.5% TTC solution: Dissolve 0.15 g of TTC (appended to the medium) in 10 mL of distilled water, and store in brown bottle in dark and cool place. Remarks The medium is used for an isolation of Enterococcus in faces, water, milk, dairy products and processed meat. Incubate at 37 °C for 48 hours. E. faecalis forms pink or red brown colonies 0.5 - 2 mm in diameter, while E. faecium forms yellow colonies. Since Enterococcus is an indicator of fecal pollution as in case of Coliform, the detection of it is important for the survey of fecal pollution. Formula HyServe GmbH & Co.KG, Hechenrainerstr. 24, 82449 Uffing, Germany; www.hyserve.com; info@hvserve.com produced by Nissui Pharmaceuticls co., ltd

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