Catalog excerpts
EMB Agar(Eosin Methylene Blue Agar) Code Directions Suspend 37.5 g of the dehydrated medium in 1,000 mL of distilled water, mix well and heat to dissolve the medium. Sterilize by autoclaving at 121 °C for 15 minutes. Distribute about 20 mL of the medium into Petri dishes. Determinations Incubate at 35 ± 1°C for 22 - 26 hours. E. coli, one of coliform organisms, forms colonies of metallic luster with a black center, while Enterobacter aerogenes forms larger colonies with a dark center. Salmonella and Shigella form colorless, transparent and small colonies. Remarks The medium is used for the differentiation of coliform organisms in foods and drinks. The growth of gram-positive organisms is inhibited by the dyes. Formula HyServe GmbH & Co.KG, Hechenrainerstr. 24, 82449 Uffing, Germany; www.hyserve.com; info@hvserve.com produced by Nissui Pharmaceuticls co., ltd
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