Catalog excerpts
MacConkey Sorbitol Agar(Isolation medium for E coli 0157) Code Directions Suspend 50.0 g of the dehydrated medium in 1,000 mL of distilled water, mix well and heat to dissolve the medium. Sterilize by autoclaving at 121 °C for 15 minutes. Distribute necessary amounts of medium into Petri dish. Remarks The medium is used for an isolation of E. coli 0157 based on the nature that 0157 does not ferment sorbitol while other E. coli do. E. coli 0157 develops a semitransparent colony after incubation at 37 °C for 18 - 20 hours. For the meanwhile other E. coli develop a redbrick color colony with light peach or redbrick zone of desoxycholic acid precipitated. Growths of gram positive coccus are inhibited. HyServe GmbH & Co.KG, Hechenrainerstr. 24, 82449 Uffing, Germany; www.hyserve.com; info@hvserve.com produced by Nissui Pharmaceuticls CO., LTD
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