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HyServe GmbH & Co. KG
Catalog excerpts
Directions Suspend 24.7 g of the dehydrated medium in 1,000 mL of distilled water, mix well and heat to dissolve the medium. Sterilize by autoclaving at 121 °C for 15 minutes. Maintain the medium at about 50 ° C. Distribute about 20 mL into Petri dishes contained the test sample in advance, mix well, and incubate at 35 - 37 ° C for 72±3 hours. Remarks Lactobacilli grown on the medium form colonies with yellowish peripheries in the depth and on the surface of the medium. As polysorbate 80 and L-cysteine are being added to the composition, the ability of the medium with supports the growth of lactobacilli has been improved. As Lactobacillus bulgaricus invariably forms yellowish colonies, its detection and determination are easy. Color of the medium may be changed to purple when incubation is prolonged, as the medium becomes alkaline due to the growth of bacteria. Bifidobacterium does not grow on the medium, and it is recommended to incubate it anaerobically with BL Agar medium. HyServe GmbH & Co.KG, Hechenrainerstr. 24, 82449 Uffing, Germany; www.hyserve.com; info@hvserve.com produced by Nissui Pharmaceuticls co., ltd
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